Development of a simple radioimmunoassay for human C3a

Herbert R. Lamche; Eva Paul; Gunther Schlag; Heinz Redl; Dale E. Hammerschmidt

doi:10.1007/BF00920078

Development of a simple radioimmunoassay for human C3a

Herbert R. Lamche, Eva Paul, Gunther Schlag, Heinz Redl, Dale E. Hammerschmidt

Medicine - Hematology, Oncology, Transplant

Research output: Contribution to journal › Article › peer-review

Abstract

A radioimmunoassay was devised for the human complement cleavage product, C3a, using charcoal separation and selective precipitation of interfering substances. When compared with the commercially available immunoassay now marketed, the assay reported here was somewhat simpler to perform; furthermore, it overcame delivery and availability problems in Europe. The assay showed a mean recovery of 87% of known amounts of C3a or C3a_desarginine and had a sensitivity of 32 ng C3a per milliliter of plasma; coefficients of variance were comparable to other radioimmunoassays in common use. Using this assay in a first clinical application, we were able to document a small but statistically significant rise in [C3a] during cardiopulmonary bypass.

Original language	English (US)
Pages (from-to)	265-276
Number of pages	12
Journal	Inflammation
Volume	12
Issue number	3
DOIs	https://doi.org/10.1007/BF00920078
State	Published - Jun 1988

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abstract = "A radioimmunoassay was devised for the human complement cleavage product, C3a, using charcoal separation and selective precipitation of interfering substances. When compared with the commercially available immunoassay now marketed, the assay reported here was somewhat simpler to perform; furthermore, it overcame delivery and availability problems in Europe. The assay showed a mean recovery of 87% of known amounts of C3a or C3adesarginine and had a sensitivity of 32 ng C3a per milliliter of plasma; coefficients of variance were comparable to other radioimmunoassays in common use. Using this assay in a first clinical application, we were able to document a small but statistically significant rise in [C3a] during cardiopulmonary bypass.",

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Development of a simple radioimmunoassay for human C3a

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