TY - JOUR
T1 - Development of Microsatellite Markers for the Apomictic Triploid Fern Myriopteris lindheimeri (Pteridaceae)
AU - Grusz, Amanda L.
AU - Pryer, Kathleen M.
N1 - Publisher Copyright:
© 2015 Grusz and Pryer.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Premise of the study: Microsatellite markers were developed for investigating the population dynamics of Myriopteris lindheimeri (Pteridaceae), an apomictic triploid fern endemic to deserts of the southwestern United States and Mexico. Methods and Results: Using 454 sequencing, 21 microsatellite markers were developed. Of these, 14 were polymorphic with up to five alleles per locus and eight markers amplified in one or more congeneric close relatives (M. covillei, M. fendleri, M. aurea, and M. rufa). To demonstrate marker utility, M. lindheimeri samples from three Arizona populations were genotyped at nine loci. For each population, diversity measures including percent polymorphic loci, frequency of heterozygotes across all loci, and genotypic diversity were calculated. Across the three populations, on average, 63% of loci were polymorphic, the average frequency of heterozygotes (across all loci) was 0.32, and average genotypic diversity was 0.34. Conclusions: These markers provide a foundation for future studies exploring polyploidy and apomixis in myriopterid ferns.
AB - Premise of the study: Microsatellite markers were developed for investigating the population dynamics of Myriopteris lindheimeri (Pteridaceae), an apomictic triploid fern endemic to deserts of the southwestern United States and Mexico. Methods and Results: Using 454 sequencing, 21 microsatellite markers were developed. Of these, 14 were polymorphic with up to five alleles per locus and eight markers amplified in one or more congeneric close relatives (M. covillei, M. fendleri, M. aurea, and M. rufa). To demonstrate marker utility, M. lindheimeri samples from three Arizona populations were genotyped at nine loci. For each population, diversity measures including percent polymorphic loci, frequency of heterozygotes across all loci, and genotypic diversity were calculated. Across the three populations, on average, 63% of loci were polymorphic, the average frequency of heterozygotes (across all loci) was 0.32, and average genotypic diversity was 0.34. Conclusions: These markers provide a foundation for future studies exploring polyploidy and apomixis in myriopterid ferns.
KW - Myriopteris lindheimeri
KW - asexual
KW - cheilanthoid
KW - polyploidy
KW - pteridophyte
KW - simple sequence repeats (SSRs)
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U2 - 10.3732/apps.1500061
DO - 10.3732/apps.1500061
M3 - Article
AN - SCOPUS:84946916327
SN - 2168-0450
VL - 3
JO - Applications in Plant Sciences
JF - Applications in Plant Sciences
IS - 11
M1 - 1500061
ER -