Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs

Ping Liu; Jinbiao Zhao; Pingting Guo; Wenqing Lu; Zhengying Geng; Crystal L. Levesque; Lee J. Johnston; Chunlin Wang; Ling Liu; Jie Zhang; Ning Ma; Shiyan Qiao; Xi Ma

doi:10.3389/fcimb.2017.00526

Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs

Ping Liu, Jinbiao Zhao, Pingting Guo, Wenqing Lu, Zhengying Geng, Crystal L. Levesque, Lee J. Johnston, Chunlin Wang, Ling Liu, Jie Zhang, Ning Ma, Shiyan Qiao, Xi Ma

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Abstract

Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.

Original language	English (US)
Article number	526
Journal	Frontiers in Cellular and Infection Microbiology
Volume	7
Issue number	DEC
DOIs	https://doi.org/10.3389/fcimb.2017.00526
State	Published - Dec 22 2017

Bibliographical note

Funding Information:
We thank Dr. Xiangli Sun, Zhixiang Sun and Yao Li in Ministry of Agriculture Feed Industry Centre, for the excellent assistance in chemical analysis. This work was supported by the National Key R&D Program of China (2017YFD0500501), College of Animal Science and Technology -Young Talents Cultivation Program- in China Agricultural University (2017DKA001), the National Natural Science Foundation of China (31722054, 31472101, and 31528018), the 111 Project (B16044), the National Department Public Benefit Research Foundation (201403047) and the developmental fund for animal science by Shenzhen Jinxinnong Feed Co., Ltd.

Publisher Copyright:
© 2017 Liu, Zhao, Guo, Lu, Geng, Levesque, Johnston, Wang, Liu, Zhang, Ma, Qiao and Ma.

Keywords

Bacillus subtilis
Cellulolytic bacteria
Corn bran
Dietary fiber
Fermentation
Gut microbiota

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Liu, P., Zhao, J., Guo, P., Lu, W., Geng, Z., Levesque, C. L., Johnston, L. J., Wang, C., Liu, L., Zhang, J., Ma, N., Qiao, S., & Ma, X. (2017). Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs. Frontiers in Cellular and Infection Microbiology, 7(DEC), Article 526. https://doi.org/10.3389/fcimb.2017.00526

Liu, P, Zhao, J, Guo, P, Lu, W, Geng, Z, Levesque, CL, Johnston, LJ, Wang, C, Liu, L, Zhang, J, Ma, N, Qiao, S & Ma, X 2017, 'Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs', Frontiers in Cellular and Infection Microbiology, vol. 7, no. DEC, 526. https://doi.org/10.3389/fcimb.2017.00526

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title = "Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs",

abstract = "Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.",

keywords = "Bacillus subtilis, Cellulolytic bacteria, Corn bran, Dietary fiber, Fermentation, Gut microbiota",

author = "Ping Liu and Jinbiao Zhao and Pingting Guo and Wenqing Lu and Zhengying Geng and Levesque, {Crystal L.} and Johnston, {Lee J.} and Chunlin Wang and Ling Liu and Jie Zhang and Ning Ma and Shiyan Qiao and Xi Ma",

note = "Funding Information: We thank Dr. Xiangli Sun, Zhixiang Sun and Yao Li in Ministry of Agriculture Feed Industry Centre, for the excellent assistance in chemical analysis. This work was supported by the National Key R&D Program of China (2017YFD0500501), College of Animal Science and Technology -Young Talents Cultivation Program- in China Agricultural University (2017DKA001), the National Natural Science Foundation of China (31722054, 31472101, and 31528018), the 111 Project (B16044), the National Department Public Benefit Research Foundation (201403047) and the developmental fund for animal science by Shenzhen Jinxinnong Feed Co., Ltd. Publisher Copyright: {\textcopyright} 2017 Liu, Zhao, Guo, Lu, Geng, Levesque, Johnston, Wang, Liu, Zhang, Ma, Qiao and Ma.",

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AU - Lu, Wenqing

AU - Geng, Zhengying

AU - Levesque, Crystal L.

AU - Johnston, Lee J.

AU - Wang, Chunlin

AU - Liu, Ling

AU - Zhang, Jie

AU - Ma, Ning

AU - Qiao, Shiyan

AU - Ma, Xi

N1 - Funding Information: We thank Dr. Xiangli Sun, Zhixiang Sun and Yao Li in Ministry of Agriculture Feed Industry Centre, for the excellent assistance in chemical analysis. This work was supported by the National Key R&D Program of China (2017YFD0500501), College of Animal Science and Technology -Young Talents Cultivation Program- in China Agricultural University (2017DKA001), the National Natural Science Foundation of China (31722054, 31472101, and 31528018), the 111 Project (B16044), the National Department Public Benefit Research Foundation (201403047) and the developmental fund for animal science by Shenzhen Jinxinnong Feed Co., Ltd. Publisher Copyright: © 2017 Liu, Zhao, Guo, Lu, Geng, Levesque, Johnston, Wang, Liu, Zhang, Ma, Qiao and Ma.

PY - 2017/12/22

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N2 - Solid-state fermentation of feedstuffs by Bacillus subtilis MA139 can reduce insoluble dietary fiber content in vitro and improve growth performance in pigs. This study was conducted to investigate the effects of dietary corn bran (CB) fermented by B. subtilis on growth performance and gut microbiota composition in finishing pigs. A total of 60 finishing pigs were allocated to 3 dietary treatments consisting of a control (CON) diet, a 10% CB diet, and a 10% fermented CB (FCB) diet in a 21 d feeding trial. Growth performance and nutrient digestibility were evaluated. Fecal samples were determined for bacterial community diversity by 16S rRNA gene amplicon sequencing. The dietary CB and FCB did not affect growth performance of finishing pigs. The digestibility of organic matter was decreased in both CB and FCB treatments compared with CON group (P < 0.05). The a-diversity for bacterial community analysis of Chao 1 in FCB treatment was lower than CON treatment (P < 0.05). The Fibrobacteres phylum belongs to cellulolytic bacteria was isolated, and their relative abundance in CB group showed no difference between CON and FCB treatments. The abundance of Lachnospiraceae_NK4A136_group in CB treatment was higher than CON and FCB groups (P < 0.05), whereas the population of norank_f_Prevotellaceae was higher in FCB group compared to CON and CB groups (P < 0.05). In conclusion, dietary FCB decreased the abundance of bacterial communities, particularly the population of bacteria related to cellulolytic degradation.

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KW - Bacillus subtilis

KW - Cellulolytic bacteria

KW - Corn bran

KW - Dietary fiber

KW - Fermentation

KW - Gut microbiota

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Dietary corn bran frmented by Bacillus subtilis MA139 decreased gut cellulolytic bacteria and microbiota diversity in finishing pigs

Abstract

Bibliographical note

Keywords

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