Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

T. Wacker; H. Jahr; S. Weinand; H. Brandhorst; D. Brandhorst; D. Lau; B. J. Hering; K. Federlin; R. G. Bretzel

doi:10.1055/s-0029-1211410

Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

T. Wacker, H. Jahr, S. Weinand, H. Brandhorst, D. Brandhorst, D. Lau, B. J. Hering, K. Federlin, R. G. Bretzel

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Abstract

Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37^°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.

Original language	English (US)
Pages (from-to)	133-135
Number of pages	3
Journal	Experimental and Clinical Endocrinology and Diabetes
Volume	103
DOIs	https://doi.org/10.1055/s-0029-1211410
State	Published - 1995

Keywords

hydrogen peroxide
islet of Langerhans
nitric oxide
superoxide

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abstract = "Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.",

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T1 - Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

AU - Wacker, T.

AU - Jahr, H.

AU - Weinand, S.

AU - Brandhorst, H.

AU - Brandhorst, D.

AU - Lau, D.

AU - Hering, B. J.

AU - Federlin, K.

AU - Bretzel, R. G.

PY - 1995

Y1 - 1995

N2 - Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.

AB - Susceptibility of islet cells to damage by hydrogen peroxide, superoxide, and nitric oxide was determined on islets isolated from humans, pigs, and rats. Islets were incubated for 20 hr at 37°C with different concentrations of hydrogen peroxide, hypoxanthine/xanthine oxidase, or nitroprusside sodium, respectively. Islet cell damage was then measured as trypan blue-uptake. Rat islets showed a higher sensitivity than human or pig islets to damage by reactive oxygen species or nitric oxide. These results indicate that pig islets may be a more suitable model than rat islets to study inflammatory islet cell damage in diabetes and clinical islet transplantation.

KW - hydrogen peroxide

KW - islet of Langerhans

KW - nitric oxide

KW - superoxide

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Different Toxic Effects of Hydrogen Peroxide, Nitric Oxide, and Superoxide on Human, Pig, and Rat Islets of Langerhans

Abstract

Keywords

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