TY - JOUR
T1 - Differentiation of avian adenovirus type-II strains by restriction endonuclease fingerprinting.
AU - Zhang, C.
AU - Nagaraja, Kakambi V
PY - 1989/9
Y1 - 1989/9
N2 - Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.
AB - Three serologically indistinguishable viruses from the avian adenovirus type-II splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. The DNA from these viruses were examined with 6 restriction endonucleases (Bgl II, EcoRI, HindIII, Hha I, Xho I, and BamHI). Markedly different DNA cleavage patterns were found in these virus isolates with all the 5 enzymes, except with BamHI, suggesting genetic differences between isolates of adenovirus type II. Restriction endonuclease analyses were found to provide a method for distinguishing genetically different, and yet serologically similar, strains of avian adenovirus type II.
UR - http://www.scopus.com/inward/record.url?scp=0024726224&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024726224&partnerID=8YFLogxK
M3 - Article
C2 - 2552877
AN - SCOPUS:0024726224
SN - 0002-9645
VL - 50
SP - 1466
EP - 1470
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 9
ER -