Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698

Jason D. Zurn; Matthew N. Rouse; Shiaoman Chao; Meriem Aoun; Godwin Macharia; Colin W. Hiebert; Zacharias A. Pretorius; J. Michael Bonman; Maricelis Acevedo

doi:10.1186/s12864-018-4438-y

Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698

Jason D. Zurn, Matthew N. Rouse, Shiaoman Chao, Meriem Aoun, Godwin Macharia, Colin W. Hiebert, Zacharias A. Pretorius, J. Michael Bonman, Maricelis Acevedo

Plant Pathology

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11 Scopus citations

Abstract

Background: Research to identify and characterize stem rust resistance genes in common wheat, Triticum aestivum, has been stimulated by the emergence of Ug99-lineage races of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), in Eastern Africa. The Montenegrin spring wheat landrace PI 362698 was identified as a source of Pgt resistance. This accession exhibits resistance to multiple Ug99-lineage and North American Pgt races at seedling and adult-plant stages. A recombinant inbred population was developed by crossing the susceptible line LMPG-6 with a single plant selection of PI 362698. A genetic map was constructed using the Illumina iSelect 90â K wheat assay and the markers csLv34, NB-LRR3, and wMAS000003 and quantitative trait locus (QTL) analysis was performed. Results: QTL analysis identified five significant QTLs (α = 0.05) on chromosomes 2B, 3B, 6A, 6D, and 7A associated with wheat stem rust resistance. The QTL on chromosome 3B was identified using both field data from Kenya (Pgt Ug99-lineage races) and seedling data from Pgt race MCCF. This QTL potentially corresponds to Sr12 or a new allele of Sr12. The multi-pathogen resistance gene Sr57 located on chromosome 7D is present in PI 362698 according to the diagnostic markers csLv34 and wMAS000003, however a significant QTL was not detected at this locus. The QTLs on chromosomes 2B, 6A, and 6D were identified during seedling trials and are thought to correspond to Sr16, Sr8a, and Sr5, respectively. The QTL identified on chromosome 7A was detected using MCCF seedling data and may be Sr15 or a potentially novel allele of recently detected Ug99 resistance QTLs. Conclusions: The combination of resistance QTLs found in PI 362698 is like the resistance gene combination present in the broadly resistant cultivar Thatcher. As such, PI 362698 may not be a landrace as previously thought. PI 362698 has been crossed with North Dakota wheat germplasm for future breeding efforts. Additional work is needed to fully understand why the combination of genes present in PI 362698 and 'Thatcher' provide such durable resistance.

Original language	English (US)
Article number	67
Journal	BMC Genomics
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12864-018-4438-y
State	Published - Jan 22 2018

Bibliographical note

Funding Information:
This project was funded through the North Dakota Wheat Commission, USDA-ARS National Plant Disease Recovery System (CRIS project 2050– 21000-029-00D), and the Delivering Genetic Gain in Wheat project. Funding bodies had no role in the design of the study, collection of data, data analysis and interpretation, and the writing of the manuscript.

Publisher Copyright:
© 2018 The Author(s).

Keywords

Food security
Infinium
KASP
Mesothetic resistance
SNP
Yield protection

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{d15e05ac8e824ce4a73b56679a25c264,

title = "Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698",

abstract = "Background: Research to identify and characterize stem rust resistance genes in common wheat, Triticum aestivum, has been stimulated by the emergence of Ug99-lineage races of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), in Eastern Africa. The Montenegrin spring wheat landrace PI 362698 was identified as a source of Pgt resistance. This accession exhibits resistance to multiple Ug99-lineage and North American Pgt races at seedling and adult-plant stages. A recombinant inbred population was developed by crossing the susceptible line LMPG-6 with a single plant selection of PI 362698. A genetic map was constructed using the Illumina iSelect 90{\^a} K wheat assay and the markers csLv34, NB-LRR3, and wMAS000003 and quantitative trait locus (QTL) analysis was performed. Results: QTL analysis identified five significant QTLs (α = 0.05) on chromosomes 2B, 3B, 6A, 6D, and 7A associated with wheat stem rust resistance. The QTL on chromosome 3B was identified using both field data from Kenya (Pgt Ug99-lineage races) and seedling data from Pgt race MCCF. This QTL potentially corresponds to Sr12 or a new allele of Sr12. The multi-pathogen resistance gene Sr57 located on chromosome 7D is present in PI 362698 according to the diagnostic markers csLv34 and wMAS000003, however a significant QTL was not detected at this locus. The QTLs on chromosomes 2B, 6A, and 6D were identified during seedling trials and are thought to correspond to Sr16, Sr8a, and Sr5, respectively. The QTL identified on chromosome 7A was detected using MCCF seedling data and may be Sr15 or a potentially novel allele of recently detected Ug99 resistance QTLs. Conclusions: The combination of resistance QTLs found in PI 362698 is like the resistance gene combination present in the broadly resistant cultivar Thatcher. As such, PI 362698 may not be a landrace as previously thought. PI 362698 has been crossed with North Dakota wheat germplasm for future breeding efforts. Additional work is needed to fully understand why the combination of genes present in PI 362698 and 'Thatcher' provide such durable resistance.",

keywords = "Food security, Infinium, KASP, Mesothetic resistance, SNP, Yield protection",

author = "Zurn, {Jason D.} and Rouse, {Matthew N.} and Shiaoman Chao and Meriem Aoun and Godwin Macharia and Hiebert, {Colin W.} and Pretorius, {Zacharias A.} and Bonman, {J. Michael} and Maricelis Acevedo",

note = "Funding Information: This project was funded through the North Dakota Wheat Commission, USDA-ARS National Plant Disease Recovery System (CRIS project 2050– 21000-029-00D), and the Delivering Genetic Gain in Wheat project. Funding bodies had no role in the design of the study, collection of data, data analysis and interpretation, and the writing of the manuscript. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = jan,

day = "22",

doi = "10.1186/s12864-018-4438-y",

language = "English (US)",

volume = "19",

journal = "BMC Genomics",

issn = "1471-2164",

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TY - JOUR

T1 - Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698

AU - Zurn, Jason D.

AU - Rouse, Matthew N.

AU - Chao, Shiaoman

AU - Aoun, Meriem

AU - Macharia, Godwin

AU - Hiebert, Colin W.

AU - Pretorius, Zacharias A.

AU - Bonman, J. Michael

AU - Acevedo, Maricelis

N1 - Funding Information: This project was funded through the North Dakota Wheat Commission, USDA-ARS National Plant Disease Recovery System (CRIS project 2050– 21000-029-00D), and the Delivering Genetic Gain in Wheat project. Funding bodies had no role in the design of the study, collection of data, data analysis and interpretation, and the writing of the manuscript. Publisher Copyright: © 2018 The Author(s).

PY - 2018/1/22

Y1 - 2018/1/22

N2 - Background: Research to identify and characterize stem rust resistance genes in common wheat, Triticum aestivum, has been stimulated by the emergence of Ug99-lineage races of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), in Eastern Africa. The Montenegrin spring wheat landrace PI 362698 was identified as a source of Pgt resistance. This accession exhibits resistance to multiple Ug99-lineage and North American Pgt races at seedling and adult-plant stages. A recombinant inbred population was developed by crossing the susceptible line LMPG-6 with a single plant selection of PI 362698. A genetic map was constructed using the Illumina iSelect 90â K wheat assay and the markers csLv34, NB-LRR3, and wMAS000003 and quantitative trait locus (QTL) analysis was performed. Results: QTL analysis identified five significant QTLs (α = 0.05) on chromosomes 2B, 3B, 6A, 6D, and 7A associated with wheat stem rust resistance. The QTL on chromosome 3B was identified using both field data from Kenya (Pgt Ug99-lineage races) and seedling data from Pgt race MCCF. This QTL potentially corresponds to Sr12 or a new allele of Sr12. The multi-pathogen resistance gene Sr57 located on chromosome 7D is present in PI 362698 according to the diagnostic markers csLv34 and wMAS000003, however a significant QTL was not detected at this locus. The QTLs on chromosomes 2B, 6A, and 6D were identified during seedling trials and are thought to correspond to Sr16, Sr8a, and Sr5, respectively. The QTL identified on chromosome 7A was detected using MCCF seedling data and may be Sr15 or a potentially novel allele of recently detected Ug99 resistance QTLs. Conclusions: The combination of resistance QTLs found in PI 362698 is like the resistance gene combination present in the broadly resistant cultivar Thatcher. As such, PI 362698 may not be a landrace as previously thought. PI 362698 has been crossed with North Dakota wheat germplasm for future breeding efforts. Additional work is needed to fully understand why the combination of genes present in PI 362698 and 'Thatcher' provide such durable resistance.

AB - Background: Research to identify and characterize stem rust resistance genes in common wheat, Triticum aestivum, has been stimulated by the emergence of Ug99-lineage races of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), in Eastern Africa. The Montenegrin spring wheat landrace PI 362698 was identified as a source of Pgt resistance. This accession exhibits resistance to multiple Ug99-lineage and North American Pgt races at seedling and adult-plant stages. A recombinant inbred population was developed by crossing the susceptible line LMPG-6 with a single plant selection of PI 362698. A genetic map was constructed using the Illumina iSelect 90â K wheat assay and the markers csLv34, NB-LRR3, and wMAS000003 and quantitative trait locus (QTL) analysis was performed. Results: QTL analysis identified five significant QTLs (α = 0.05) on chromosomes 2B, 3B, 6A, 6D, and 7A associated with wheat stem rust resistance. The QTL on chromosome 3B was identified using both field data from Kenya (Pgt Ug99-lineage races) and seedling data from Pgt race MCCF. This QTL potentially corresponds to Sr12 or a new allele of Sr12. The multi-pathogen resistance gene Sr57 located on chromosome 7D is present in PI 362698 according to the diagnostic markers csLv34 and wMAS000003, however a significant QTL was not detected at this locus. The QTLs on chromosomes 2B, 6A, and 6D were identified during seedling trials and are thought to correspond to Sr16, Sr8a, and Sr5, respectively. The QTL identified on chromosome 7A was detected using MCCF seedling data and may be Sr15 or a potentially novel allele of recently detected Ug99 resistance QTLs. Conclusions: The combination of resistance QTLs found in PI 362698 is like the resistance gene combination present in the broadly resistant cultivar Thatcher. As such, PI 362698 may not be a landrace as previously thought. PI 362698 has been crossed with North Dakota wheat germplasm for future breeding efforts. Additional work is needed to fully understand why the combination of genes present in PI 362698 and 'Thatcher' provide such durable resistance.

KW - Food security

KW - Infinium

KW - KASP

KW - Mesothetic resistance

KW - SNP

KW - Yield protection

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SN - 1471-2164

VL - 19

JO - BMC Genomics

JF - BMC Genomics

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ER -

Dissection of the multigenic wheat stem rust resistance present in the Montenegrin spring wheat accession PI 362698

Abstract

Bibliographical note

Keywords

UN SDGs

Access

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