Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

Mirian F. Pimentel; Ali Y. Srour; Amanda J. Warner; Jason P. Bond; Carl A. Bradley; John Rupe; Martin I. Chilvers; J. Alejandro Rojas; Janette L. Jacobs; Christopher R. Little; Alison E. Robertson; Loren J. Giesler; Dean Malvick; Kiersten Wise; Albert Tenuta; Ahmad M. Fakhoury

doi:10.1111/jam.15507

Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

Mirian F. Pimentel, Ali Y. Srour, Amanda J. Warner, Jason P. Bond, Carl A. Bradley, John Rupe, Martin I. Chilvers, J. Alejandro Rojas, Janette L. Jacobs, Christopher R. Little, Alison E. Robertson, Loren J. Giesler, Dean Malvick, Kiersten Wise, Albert Tenuta, Ahmad M. Fakhoury

Plant Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence. Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. Most recovered species are known soybean pathogens. However, non-pathogenic organisms were also isolated. Crop history, soil density, water source, precipitation and temperature were the main factors influencing the abundance of fungal species. Conclusion: Key fungal species associated with soybean seedling diseases occurring in several US production regions were characterized. This work also identified major environment and edaphic factors affecting the abundance and occurrence of these species. Significance and Impact of the Study: The identification and characterization of the main pathogens associated with seedling diseases across major soybean-producing areas could help manage those pathogens, and devise more effective and sustainable practices to reduce the damage they cause.

Original language	English (US)
Pages (from-to)	3797-3811
Number of pages	15
Journal	Journal of Applied Microbiology
Volume	132
Issue number	5
DOIs	https://doi.org/10.1111/jam.15507
State	Published - May 2022

Bibliographical note

Funding Information:
This research was funded through a grant from the United Soybean Board (USB) and the North Central Soybean Research Program (NCSRP). Thanks for the hard work of all staff involved sampling, fungal isolation, and shipping to SIUC: T. Welacky (Ontario), T. Anderson (Ontario), C. Van Herk (Ontario), G. Kotulak (Ontario), G. Stasko (Ontario), A. Steger (Arkansas), R. Holland (Akansas), K. Urrea (Akansas), N. Anderson (Indiana), J. Ravellette (Indiana), R. Matthiesen (Iowa), V. Silva (Iowa), D. Pedersen (Illinois), U. Reuter-Carlson (Illinois), J. O’Mara (Kansas), B. Grabow (Kansas), B. McKinzie (Kansas), C. Floyd (Minnesota) and C. Groves (Wisconsin).

Funding Information:
This research was funded through a grant from the United Soybean Board (USB) and the North Central Soybean Research Program (NCSRP). Thanks for the hard work of all staff involved sampling, fungal isolation, and shipping to SIUC: T. Welacky (Ontario), T. Anderson (Ontario), C. Van Herk (Ontario), G. Kotulak (Ontario), G. Stasko (Ontario), A. Steger (Arkansas), R. Holland (Akansas), K. Urrea (Akansas), N. Anderson (Indiana), J. Ravellette (Indiana), R. Matthiesen (Iowa), V. Silva (Iowa), D. Pedersen (Illinois), U. Reuter‐Carlson (Illinois), J. O’Mara (Kansas), B. Grabow (Kansas), B. McKinzie (Kansas), C. Floyd (Minnesota) and C. Groves (Wisconsin).

Publisher Copyright:
© 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.

Keywords

Fusarium spp.
Glycine max
Trichoderma spp.
environmental factors
seedling diseases
soilborne pathogens

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Pimentel, M. F., Srour, A. Y., Warner, A. J., Bond, J. P., Bradley, C. A., Rupe, J., Chilvers, M. I., Rojas, J. A., Jacobs, J. L., Little, C. R., Robertson, A. E., Giesler, L. J., Malvick, D., Wise, K., Tenuta, A., & Fakhoury, A. M. (2022). Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States. Journal of Applied Microbiology, 132(5), 3797-3811. https://doi.org/10.1111/jam.15507

Pimentel, MF, Srour, AY, Warner, AJ, Bond, JP, Bradley, CA, Rupe, J, Chilvers, MI, Rojas, JA, Jacobs, JL, Little, CR, Robertson, AE, Giesler, LJ, Malvick, D, Wise, K, Tenuta, A & Fakhoury, AM 2022, 'Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States', Journal of Applied Microbiology, vol. 132, no. 5, pp. 3797-3811. https://doi.org/10.1111/jam.15507

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title = "Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States",

abstract = "Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence. Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. Most recovered species are known soybean pathogens. However, non-pathogenic organisms were also isolated. Crop history, soil density, water source, precipitation and temperature were the main factors influencing the abundance of fungal species. Conclusion: Key fungal species associated with soybean seedling diseases occurring in several US production regions were characterized. This work also identified major environment and edaphic factors affecting the abundance and occurrence of these species. Significance and Impact of the Study: The identification and characterization of the main pathogens associated with seedling diseases across major soybean-producing areas could help manage those pathogens, and devise more effective and sustainable practices to reduce the damage they cause.",

keywords = "Fusarium spp., Glycine max, Trichoderma spp., environmental factors, seedling diseases, soilborne pathogens",

author = "Pimentel, {Mirian F.} and Srour, {Ali Y.} and Warner, {Amanda J.} and Bond, {Jason P.} and Bradley, {Carl A.} and John Rupe and Chilvers, {Martin I.} and Rojas, {J. Alejandro} and Jacobs, {Janette L.} and Little, {Christopher R.} and Robertson, {Alison E.} and Giesler, {Loren J.} and Dean Malvick and Kiersten Wise and Albert Tenuta and Fakhoury, {Ahmad M.}",

note = "Funding Information: This research was funded through a grant from the United Soybean Board (USB) and the North Central Soybean Research Program (NCSRP). Thanks for the hard work of all staff involved sampling, fungal isolation, and shipping to SIUC: T. Welacky (Ontario), T. Anderson (Ontario), C. Van Herk (Ontario), G. Kotulak (Ontario), G. Stasko (Ontario), A. Steger (Arkansas), R. Holland (Akansas), K. Urrea (Akansas), N. Anderson (Indiana), J. Ravellette (Indiana), R. Matthiesen (Iowa), V. Silva (Iowa), D. Pedersen (Illinois), U. Reuter-Carlson (Illinois), J. O{\textquoteright}Mara (Kansas), B. Grabow (Kansas), B. McKinzie (Kansas), C. Floyd (Minnesota) and C. Groves (Wisconsin). Funding Information: This research was funded through a grant from the United Soybean Board (USB) and the North Central Soybean Research Program (NCSRP). Thanks for the hard work of all staff involved sampling, fungal isolation, and shipping to SIUC: T. Welacky (Ontario), T. Anderson (Ontario), C. Van Herk (Ontario), G. Kotulak (Ontario), G. Stasko (Ontario), A. Steger (Arkansas), R. Holland (Akansas), K. Urrea (Akansas), N. Anderson (Indiana), J. Ravellette (Indiana), R. Matthiesen (Iowa), V. Silva (Iowa), D. Pedersen (Illinois), U. Reuter‐Carlson (Illinois), J. O{\textquoteright}Mara (Kansas), B. Grabow (Kansas), B. McKinzie (Kansas), C. Floyd (Minnesota) and C. Groves (Wisconsin). Publisher Copyright: {\textcopyright} 2022 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.",

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doi = "10.1111/jam.15507",

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T1 - Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

AU - Pimentel, Mirian F.

AU - Srour, Ali Y.

AU - Warner, Amanda J.

AU - Bond, Jason P.

AU - Bradley, Carl A.

AU - Rupe, John

AU - Chilvers, Martin I.

AU - Rojas, J. Alejandro

AU - Jacobs, Janette L.

AU - Little, Christopher R.

AU - Robertson, Alison E.

AU - Giesler, Loren J.

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AU - Wise, Kiersten

AU - Tenuta, Albert

AU - Fakhoury, Ahmad M.

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N2 - Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence. Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. Most recovered species are known soybean pathogens. However, non-pathogenic organisms were also isolated. Crop history, soil density, water source, precipitation and temperature were the main factors influencing the abundance of fungal species. Conclusion: Key fungal species associated with soybean seedling diseases occurring in several US production regions were characterized. This work also identified major environment and edaphic factors affecting the abundance and occurrence of these species. Significance and Impact of the Study: The identification and characterization of the main pathogens associated with seedling diseases across major soybean-producing areas could help manage those pathogens, and devise more effective and sustainable practices to reduce the damage they cause.

AB - Aims: To isolate and characterize fungi associated with diseased soybean seedlings in Midwestern soybean production fields and to determine the influence of environmental and edaphic factors on their incidence. Methods and Results: Seedlings were collected from fields with seedling disease history in 2012 and 2013 for fungal isolation. Environmental and edaphic data associated with each field was collected. 3036 fungal isolates were obtained and assigned to 76 species. The most abundant genera recovered were Fusarium (73%) and Trichoderma (11.2%). Other genera included Mortierella, Clonostachys, Rhizoctonia, Alternaria, Mucor, Phoma, Macrophomina and Phomopsis. Most recovered species are known soybean pathogens. However, non-pathogenic organisms were also isolated. Crop history, soil density, water source, precipitation and temperature were the main factors influencing the abundance of fungal species. Conclusion: Key fungal species associated with soybean seedling diseases occurring in several US production regions were characterized. This work also identified major environment and edaphic factors affecting the abundance and occurrence of these species. Significance and Impact of the Study: The identification and characterization of the main pathogens associated with seedling diseases across major soybean-producing areas could help manage those pathogens, and devise more effective and sustainable practices to reduce the damage they cause.

KW - Fusarium spp.

KW - Glycine max

KW - Trichoderma spp.

KW - environmental factors

KW - seedling diseases

KW - soilborne pathogens

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Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

Access

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