Effect of Mobile Phase Composition and Highly Fluorinated Bonded Phases on the Apparent Free Energy of Transfer of Solute Functional Groups

The purpose of the present work was to study in detail the chromatographic characteristics of three types of highly fluorinated bonded-phase columns and one analogous hydrocarbonaceous phase in a variety of methanol/water mobile phases. The use of a 42-member, multifunctional solute set enabled the overall direct comparison of these four bonded phases. Particular attention was given to the characterization of the analogous fluorlnated/hydrocarbon bonded phases. These two columns were studied with respect to their retention of members of an homologous series (i.e., through a methylene increment) and a wide variety of functional groups and their overall chromatographic stability In a strongly basic mobile phase (0.01 M sodium hydroxide In 50/50 (v/v) methanol/water).