TY - JOUR
T1 - Effects of Dentin Debris on the Antimicrobial Properties of Sodium Hypochlorite and Etidronic Acid
AU - Arias-Moliz, Maria Teresa
AU - Morago, Ana
AU - Ordinola-Zapata, Ronald
AU - Ferrer-Luque, Carmen María
AU - Ruiz-Linares, Matilde
AU - Baca, Pilar
N1 - Funding Information:
Supported by the Research Group CTS-167 (Junta de Andalucía, Spain).
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Introduction The purpose of this study was to determine the influence of dentin powder on the concentration, pH, and antimicrobial activity of sodium hypochlorite (NaOCl) alone and combined with etidronic acid (HEBP). Methods Biofilms of Enterococcus faecalis were grown on the surface of dentin blocks for 5 days and then exposed to 1% and 2.5% NaOCl alone or combined with 9% HEBP for 3 minutes in the absence and presence of dentin powder. The biovolumes of the biofilm were measured using confocal microscopy and the live/dead technique. The available chlorine and pH of the solutions were also measured. Nonparametric tests were used to determine statistical differences (P <.05). Results The presence of dentin powder resulted in a reduction of the free available chlorine and pH in all the irrigating solutions; 1% NaOCl lost its antimicrobial activity completely in the presence of dentin powder. The antimicrobial activity was significantly reduced in the 2.5% NaOCl and 1% NaOCl/HEBP groups, and it was not affected in the 2.5% NaOCl/HEBP group. Conclusions The presence of dentin powder significantly decreased the available chlorine and antimicrobial activity of 1% NaOCl, 2.5% NaOCl, and 1% NaOCl/HEBP irrigating solutions. The antimicrobial activity of 2.5% NaOCl/HEBP was not affected by the dentin powder after a 3-minute contact time against E. faecalis biofilms.
AB - Introduction The purpose of this study was to determine the influence of dentin powder on the concentration, pH, and antimicrobial activity of sodium hypochlorite (NaOCl) alone and combined with etidronic acid (HEBP). Methods Biofilms of Enterococcus faecalis were grown on the surface of dentin blocks for 5 days and then exposed to 1% and 2.5% NaOCl alone or combined with 9% HEBP for 3 minutes in the absence and presence of dentin powder. The biovolumes of the biofilm were measured using confocal microscopy and the live/dead technique. The available chlorine and pH of the solutions were also measured. Nonparametric tests were used to determine statistical differences (P <.05). Results The presence of dentin powder resulted in a reduction of the free available chlorine and pH in all the irrigating solutions; 1% NaOCl lost its antimicrobial activity completely in the presence of dentin powder. The antimicrobial activity was significantly reduced in the 2.5% NaOCl and 1% NaOCl/HEBP groups, and it was not affected in the 2.5% NaOCl/HEBP group. Conclusions The presence of dentin powder significantly decreased the available chlorine and antimicrobial activity of 1% NaOCl, 2.5% NaOCl, and 1% NaOCl/HEBP irrigating solutions. The antimicrobial activity of 2.5% NaOCl/HEBP was not affected by the dentin powder after a 3-minute contact time against E. faecalis biofilms.
KW - Antimicrobial activity
KW - dentin debris
KW - etidronic acid
KW - root canal irrigants
KW - sodium hypochlorite
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U2 - 10.1016/j.joen.2016.01.021
DO - 10.1016/j.joen.2016.01.021
M3 - Article
C2 - 26951957
AN - SCOPUS:84959527363
SN - 0099-2399
VL - 42
SP - 771
EP - 775
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 5
ER -