Abstract
Affinity-based cell separation is label-free and highly specific, but it is difficult to efficiently and gently release affinity-captured cells due to the multivalent nature of cell-material interactions. To address this challenge, we have developed a platform composed of a capture substrate and a cell-releasing molecular trigger. The capture substrate is functionalized with a cell-capture antibody and a coiled-coil A. The cell-releasing molecular trigger B-PEG (polyethylene glycol), a conjugate of a coiled-coil B and polyethylene glycol, can drive efficient and gentle release of the captured cells, because A/B heterodimerization brings B-PEG to the substrate and PEG chains adopt extended conformations and break nearby multivalent antibody-biomarker interactions. No enzymes or excessive shear stress are involved, and the released cells have neither external molecules attached nor endogenous cell-surface molecules cleaved, which is critical for the viability, phenotype, and function of sensitive cells. (Figure presented.).
Original language | English (US) |
---|---|
Article number | 1600330 |
Journal | Macromolecular Bioscience |
Volume | 17 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 2017 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords
- affinity-capture
- cell release
- cell separation
- coiled-coil
- self-assembling