Endogenous expression of nNOS protein in several neuronal cell lines

Marianne K O Grant; Adolfo E. Cuadra; Esam E El-Fakahany

doi:10.1016/S0024-3205(02)01732-0

Endogenous expression of nNOS protein in several neuronal cell lines

Marianne K O Grant, Adolfo E. Cuadra, Esam E El-Fakahany

Experimental and Clinical Pharmacology

Research output: Contribution to journal › Article › peer-review

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Abstract

Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma × glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells.

Original language	English (US)
Pages (from-to)	813-817
Number of pages	5
Journal	Life Sciences
Volume	71
Issue number	7
DOIs	https://doi.org/10.1016/S0024-3205(02)01732-0
State	Published - Jul 5 2002

Bibliographical note

Funding Information:
This work was supported by NIH grant NS25743.

Keywords

Neuroblastoma cells
Neuronal nitric oxide synthase
Western blot analysis

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title = "Endogenous expression of nNOS protein in several neuronal cell lines",

abstract = "Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma × glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells.",

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author = "Grant, {Marianne K O} and Cuadra, {Adolfo E.} and El-Fakahany, {Esam E}",

note = "Funding Information: This work was supported by NIH grant NS25743.",

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doi = "10.1016/S0024-3205(02)01732-0",

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AU - Cuadra, Adolfo E.

AU - El-Fakahany, Esam E

N1 - Funding Information: This work was supported by NIH grant NS25743.

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AB - Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma × glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells.

KW - Neuroblastoma cells

KW - Neuronal nitric oxide synthase

KW - Western blot analysis

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Endogenous expression of nNOS protein in several neuronal cell lines

Abstract

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Keywords

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