ENFET glucose biosensor produced with mesoporous silica microspheres

Ke Yao; Yihua Zhu; Ping Wang; Xiaoling Yang; Pengzhi Cheng; Hui Lu

doi:10.1016/j.msec.2006.07.011

ENFET glucose biosensor produced with mesoporous silica microspheres

Ke Yao, Yihua Zhu, Ping Wang, Xiaoling Yang, Pengzhi Cheng, Hui Lu

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The biosensors based on ENFETs for direct glucose oxidase (GOD) concentration analysis have been fabricated. Mesoporous silica (MS) microspheres of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS microspheres show significantly improved enzyme immobilization capacity. The glucose sensitive ENFET based on the modification of the gate surface of ion-sensitive field-effect transistors (ISFETs) with MS microspheres and glucose oxidase (GOD) shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. MS microspheres of different pore sizes were investigated and the sensor with remodeled MS microspheres which experienced the pore expanding treatment show better performance than untreated MS microspheres. The fabricated sensors have a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.10 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability and reproducibility.

Original language	English (US)
Pages (from-to)	736-740
Number of pages	5
Journal	Materials Science and Engineering C
Volume	27
Issue number	4
DOIs	https://doi.org/10.1016/j.msec.2006.07.011
State	Published - May 16 2007

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (20236020, 20276019), the Shanghai Municipal Science and Technology Commission (04DZ14002, 04DZ05622, 05DZ22302).

Keywords

Biosensor
ENFET
Glucose
Mesoporous silica microspheres

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title = "ENFET glucose biosensor produced with mesoporous silica microspheres",

abstract = "The biosensors based on ENFETs for direct glucose oxidase (GOD) concentration analysis have been fabricated. Mesoporous silica (MS) microspheres of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS microspheres show significantly improved enzyme immobilization capacity. The glucose sensitive ENFET based on the modification of the gate surface of ion-sensitive field-effect transistors (ISFETs) with MS microspheres and glucose oxidase (GOD) shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. MS microspheres of different pore sizes were investigated and the sensor with remodeled MS microspheres which experienced the pore expanding treatment show better performance than untreated MS microspheres. The fabricated sensors have a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.10 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability and reproducibility.",

keywords = "Biosensor, ENFET, Glucose, Mesoporous silica microspheres",

author = "Ke Yao and Yihua Zhu and Ping Wang and Xiaoling Yang and Pengzhi Cheng and Hui Lu",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (20236020, 20276019), the Shanghai Municipal Science and Technology Commission (04DZ14002, 04DZ05622, 05DZ22302).",

year = "2007",

month = may,

day = "16",

doi = "10.1016/j.msec.2006.07.011",

language = "English (US)",

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T1 - ENFET glucose biosensor produced with mesoporous silica microspheres

AU - Yao, Ke

AU - Zhu, Yihua

AU - Wang, Ping

AU - Yang, Xiaoling

AU - Cheng, Pengzhi

AU - Lu, Hui

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (20236020, 20276019), the Shanghai Municipal Science and Technology Commission (04DZ14002, 04DZ05622, 05DZ22302).

PY - 2007/5/16

Y1 - 2007/5/16

N2 - The biosensors based on ENFETs for direct glucose oxidase (GOD) concentration analysis have been fabricated. Mesoporous silica (MS) microspheres of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS microspheres show significantly improved enzyme immobilization capacity. The glucose sensitive ENFET based on the modification of the gate surface of ion-sensitive field-effect transistors (ISFETs) with MS microspheres and glucose oxidase (GOD) shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. MS microspheres of different pore sizes were investigated and the sensor with remodeled MS microspheres which experienced the pore expanding treatment show better performance than untreated MS microspheres. The fabricated sensors have a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.10 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability and reproducibility.

AB - The biosensors based on ENFETs for direct glucose oxidase (GOD) concentration analysis have been fabricated. Mesoporous silica (MS) microspheres of different pore sizes were used as the substrates for immobilizing enzyme to construct ENFETs. The MS microspheres show significantly improved enzyme immobilization capacity. The glucose sensitive ENFET based on the modification of the gate surface of ion-sensitive field-effect transistors (ISFETs) with MS microspheres and glucose oxidase (GOD) shows obviously enhanced sensitivity and extended lifetime compared with the conventional ones. MS microspheres of different pore sizes were investigated and the sensor with remodeled MS microspheres which experienced the pore expanding treatment show better performance than untreated MS microspheres. The fabricated sensors have a linear range of 0.25-2.0 mM, and a detection limit of ca. 0.10 mM. The influence of buffer concentration, ionic strength and pH was discussed. The biosensor also has good stability and reproducibility.

KW - Biosensor

KW - ENFET

KW - Glucose

KW - Mesoporous silica microspheres

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ER -

ENFET glucose biosensor produced with mesoporous silica microspheres

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