TY - JOUR
T1 - Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome
AU - Shchukina, Irina
AU - Bagaitkar, Juhi
AU - Shpynov, Oleg
AU - Loginicheva, Ekaterina
AU - Porter, Sofia
AU - Mogilenko, Denis A.
AU - Wolin, Erica
AU - Collins, Patrick
AU - Demidov, German
AU - Artomov, Mykyta
AU - Zaitsev, Konstantin
AU - Sidorov, Sviatoslav
AU - Camell, Christina
AU - Bambouskova, Monika
AU - Arthur, Laura
AU - Swain, Amanda
AU - Panteleeva, Alexandra
AU - Dievskii, Aleksei
AU - Kurbatsky, Evgeny
AU - Tsurinov, Petr
AU - Chernyatchik, Roman
AU - Dixit, Vishwa Deep
AU - Jovanovic, Marko
AU - Stewart, Sheila A.
AU - Daly, Mark J.
AU - Dmitriev, Sergey
AU - Oltz, Eugene M.
AU - Artyomov, Maxim N.
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.
PY - 2021/1
Y1 - 2021/1
N2 - The impact of healthy aging on molecular programming of immune cells is poorly understood. Here we report comprehensive characterization of healthy aging in human classical monocytes, with a focus on epigenomic, transcriptomic and proteomic alterations, as well as the corresponding proteomic and metabolomic data for plasma, using healthy cohorts of 20 young and 20 older males (~27 and ~64 years old on average). For each individual, we performed enhanced reduced representation bisulfite sequencing-based DNA methylation profiling, which allowed us to identify a set of age-associated differentially methylated regions (DMRs)—a novel, cell-type-specific signature of aging in the DNA methylome. Hypermethylation events were associated with H3K27me3 in the CpG islands near promoters of lowly expressed genes, while hypomethylated DMRs were enriched in H3K4me1-marked regions and associated with age-related increase of expression of the corresponding genes, providing a link between DNA methylation and age-associated transcriptional changes in primary human cells.
AB - The impact of healthy aging on molecular programming of immune cells is poorly understood. Here we report comprehensive characterization of healthy aging in human classical monocytes, with a focus on epigenomic, transcriptomic and proteomic alterations, as well as the corresponding proteomic and metabolomic data for plasma, using healthy cohorts of 20 young and 20 older males (~27 and ~64 years old on average). For each individual, we performed enhanced reduced representation bisulfite sequencing-based DNA methylation profiling, which allowed us to identify a set of age-associated differentially methylated regions (DMRs)—a novel, cell-type-specific signature of aging in the DNA methylome. Hypermethylation events were associated with H3K27me3 in the CpG islands near promoters of lowly expressed genes, while hypomethylated DMRs were enriched in H3K4me1-marked regions and associated with age-related increase of expression of the corresponding genes, providing a link between DNA methylation and age-associated transcriptional changes in primary human cells.
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U2 - 10.1038/s43587-020-00002-6
DO - 10.1038/s43587-020-00002-6
M3 - Article
C2 - 34796338
AN - SCOPUS:85101520958
SN - 2662-8465
VL - 1
SP - 124
EP - 141
JO - Nature Aging
JF - Nature Aging
IS - 1
ER -
