TY - JOUR
T1 - Enhanced potency of covalently modified granulocyte colony-stimulating factor
AU - Sarkar, Casim
AU - Wang, Peggy
AU - Lauffenburger, Douglas
PY - 2000/12/1
Y1 - 2000/12/1
N2 - We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.
AB - We have measured the degradation of GCSF and a PEGylated GCSF analog (SD/01) over time in vitro, and both fit a first-order depletion model. We found that SD/01 has a 5-fold longer half-life than GCSF at starting concentrations of 270 pM. However, an increase in the cellular response due to SD/01 was not noticeable until after the fifth day. These results suggest that GCSF and SD/01 may have different trafficking properties.
UR - http://www.scopus.com/inward/record.url?scp=0034498489&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034498489&partnerID=8YFLogxK
M3 - Conference article
AN - SCOPUS:0034498489
SN - 0090-6964
VL - 28
SP - S-83
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - SUPPL. 1
T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society
Y2 - 12 October 2000 through 14 October 2000
ER -