TY - JOUR
T1 - Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection
AU - Keogh, Damien
AU - Tay, Wei Hong
AU - Ho, Yao Yong
AU - Dale, Jennifer L
AU - Chen, Siyi
AU - Umashankar, Shivshankar
AU - Williams, Rohan B.H.
AU - Chen, Swaine L.
AU - Dunny, Gary M
AU - Kline, Kimberly A.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/10/12
Y1 - 2016/10/12
N2 - Enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. E. faecalis co-exists with Escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. During infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. In this study, we investigated the contribution of E. faecalis to mixed-species infection when iron availability is restricted. We show that E. faecalis significantly augments E. coli biofilm growth and survival in vitro and in vivo by exporting L-ornithine. This metabolic cue facilitates E. coli biosynthesis of the enterobactin siderophore, allowing E. coli growth and biofilm formation in iron-limiting conditions that would otherwise restrict its growth. Thus, E. faecalis modulates its local environment by contributing growth-promoting cues that allow co-infecting organisms to overcome iron limitation and promotes polymicrobial infections.
AB - Enterococcus faecalis is frequently associated with polymicrobial infections of the urinary tract, indwelling catheters, and surgical wound sites. E. faecalis co-exists with Escherichia coli and other pathogens in wound infections, but mechanisms that govern polymicrobial colonization and pathogenesis are poorly defined. During infection, bacteria must overcome multiple host defenses, including nutrient iron limitation, to persist and cause disease. In this study, we investigated the contribution of E. faecalis to mixed-species infection when iron availability is restricted. We show that E. faecalis significantly augments E. coli biofilm growth and survival in vitro and in vivo by exporting L-ornithine. This metabolic cue facilitates E. coli biosynthesis of the enterobactin siderophore, allowing E. coli growth and biofilm formation in iron-limiting conditions that would otherwise restrict its growth. Thus, E. faecalis modulates its local environment by contributing growth-promoting cues that allow co-infecting organisms to overcome iron limitation and promotes polymicrobial infections.
KW - Enterococcus faecalis
KW - Escherichia coli
KW - iron
KW - nutritional immunity
KW - polymicrobial infection
KW - wound infection
UR - http://www.scopus.com/inward/record.url?scp=84992437277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84992437277&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2016.09.004
DO - 10.1016/j.chom.2016.09.004
M3 - Article
C2 - 27736645
AN - SCOPUS:84992437277
SN - 1931-3128
VL - 20
SP - 493
EP - 503
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -