TY - JOUR
T1 - Establishment and characterization of two novel patient-derived lines from canine high-grade glioma
AU - Schrock, Morgan S.
AU - Zalenski, Abigail A.
AU - Tallman, Miranda M.
AU - Kollin, Luke
AU - Bratasz, Anna
AU - Weeks, Griffin
AU - Miller, Margaret A.
AU - Sweeney, Courtney N.
AU - Pluhar, G. Elizabeth
AU - Olin, Michael R.
AU - Kisseberth, William C.
AU - Bentley, R. Timothy
AU - Dickinson, Peter J.
AU - York, Daniel
AU - Webb, Amy
AU - Wang, Xu
AU - Moore, Sarah
AU - Venere, Monica
AU - Summers, Matthew K.
N1 - Publisher Copyright:
© 2023 The Authors. Veterinary and Comparative Oncology published by John Wiley & Sons Ltd.
PY - 2023/9
Y1 - 2023/9
N2 - High-grade glioma is an aggressive cancer that occurs naturally in pet dogs. Canine high-grade glioma (cHGG) is treated with radiation, chemotherapy or surgery, but has no curative treatment. Within the past eight years, there have been advances in our imaging and histopathology standards as well as genetic charactereization of cHGG. However, there are only three cHGG cell lines publicly available, all of which were derived from astrocytoma and established using methods involving expansion of tumour cells in vitro on plastic dishes. In order to provide more clinically relevant cell lines for studying cHGG in vitro, the goal of this study was to establish cHGG patient-derived lines, whereby cancer cells are expanded in vivo by injecting cells into immunocompromized laboratory mice. The cells are then harvested from mice and used for in vitro studies. This method is the standard in the human field and has been shown to minimize the acquisition of genetic alterations and gene expression changes from the original tumour. Through a multi-institutional collaboration, we describe our methods for establishing two novel cHGG patient-derived lines, Boo-HA and Mo-HO, from a high-grade astrocytoma and a high-grade oligodendroglioma, respectively. We compare our novel lines to G06-A, J3T-Bg, and SDT-3G (traditional cHGG cell lines) in terms of proliferation and sensitivity to radiation. We also perform whole genome sequencing and identify an NF1 truncating mutation in Mo-HO. We report the characterization and availability of these novel patient-derived lines for use by the veterinary community.
AB - High-grade glioma is an aggressive cancer that occurs naturally in pet dogs. Canine high-grade glioma (cHGG) is treated with radiation, chemotherapy or surgery, but has no curative treatment. Within the past eight years, there have been advances in our imaging and histopathology standards as well as genetic charactereization of cHGG. However, there are only three cHGG cell lines publicly available, all of which were derived from astrocytoma and established using methods involving expansion of tumour cells in vitro on plastic dishes. In order to provide more clinically relevant cell lines for studying cHGG in vitro, the goal of this study was to establish cHGG patient-derived lines, whereby cancer cells are expanded in vivo by injecting cells into immunocompromized laboratory mice. The cells are then harvested from mice and used for in vitro studies. This method is the standard in the human field and has been shown to minimize the acquisition of genetic alterations and gene expression changes from the original tumour. Through a multi-institutional collaboration, we describe our methods for establishing two novel cHGG patient-derived lines, Boo-HA and Mo-HO, from a high-grade astrocytoma and a high-grade oligodendroglioma, respectively. We compare our novel lines to G06-A, J3T-Bg, and SDT-3G (traditional cHGG cell lines) in terms of proliferation and sensitivity to radiation. We also perform whole genome sequencing and identify an NF1 truncating mutation in Mo-HO. We report the characterization and availability of these novel patient-derived lines for use by the veterinary community.
KW - canine brain cancer
KW - canine high-grade glioma
KW - canine oligodendroglioma
KW - patient-derived line
KW - radiation
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U2 - 10.1111/vco.12912
DO - 10.1111/vco.12912
M3 - Article
C2 - 37254642
AN - SCOPUS:85161335736
SN - 1476-5810
VL - 21
SP - 492
EP - 502
JO - Veterinary and Comparative Oncology
JF - Veterinary and Comparative Oncology
IS - 3
ER -