Expression of cardiac neural crest and heart genes isolated by modified differential display

Brad J. Martinsen; Nathan J. Groebner; Allison J. Frasier; Jamie L Lohr

doi:10.1016/S1567-133X(03)00088-7

Expression of cardiac neural crest and heart genes isolated by modified differential display

Brad J. Martinsen, Nathan J. Groebner, Allison J. Frasier, Jamie L Lohr

Pediatrics - Pediatric Cardiology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The invasion of the cardiac neural crest (CNC) into the outflow tract (OFT) and subsequent outflow tract septation are critical events during vertebrate heart development. We have performed four modified differential display screens in the chick embryo to identify genes that may be involved in CNC, OFT, secondary heart field, and heart development. The screens included differential display of RNA isolated from three different axial segments containing premigratory cranial neural crest cells; of RNA from distal outflow tract, proximal outflow tract, and atrioventricular tissue of embryonic chick hearts; and of RNA isolated from left and right cranial tissues, including the early heart fields. These screens have resulted in the identification of the five cDNA clones presented here, which are expressed in the cardiac neural crest, outflow tract and developing heart in patterns that are unique in heart development.

Original language	English (US)
Pages (from-to)	407-411
Number of pages	5
Journal	Gene Expression Patterns
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/S1567-133X(03)00088-7
State	Published - Aug 2003

Bibliographical note

Funding Information:
This work was supported by an American Heart Association Fellowship (#0020507Z) to BJM; a Minnesota Medical Foundation Grant and Lillehei Heart Institute Fellowship to NJG; and a gift from the Andrew David Sit Foundation to JLL.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Keywords

Cardiac chamber specification
Cardiac inflow
Cardiac neural crest
Cardiac valve formation
Chick
Congenital heart disease
Differential display
Differential expression
Heart development
Linear heart tube
Neural crest
Notochord
Outflow tract septation
Secondary heart field

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title = "Expression of cardiac neural crest and heart genes isolated by modified differential display",

abstract = "The invasion of the cardiac neural crest (CNC) into the outflow tract (OFT) and subsequent outflow tract septation are critical events during vertebrate heart development. We have performed four modified differential display screens in the chick embryo to identify genes that may be involved in CNC, OFT, secondary heart field, and heart development. The screens included differential display of RNA isolated from three different axial segments containing premigratory cranial neural crest cells; of RNA from distal outflow tract, proximal outflow tract, and atrioventricular tissue of embryonic chick hearts; and of RNA isolated from left and right cranial tissues, including the early heart fields. These screens have resulted in the identification of the five cDNA clones presented here, which are expressed in the cardiac neural crest, outflow tract and developing heart in patterns that are unique in heart development.",

keywords = "Cardiac chamber specification, Cardiac inflow, Cardiac neural crest, Cardiac valve formation, Chick, Congenital heart disease, Differential display, Differential expression, Heart development, Linear heart tube, Neural crest, Notochord, Outflow tract septation, Secondary heart field",

author = "Martinsen, {Brad J.} and Groebner, {Nathan J.} and Frasier, {Allison J.} and Lohr, {Jamie L}",

note = "Funding Information: This work was supported by an American Heart Association Fellowship (#0020507Z) to BJM; a Minnesota Medical Foundation Grant and Lillehei Heart Institute Fellowship to NJG; and a gift from the Andrew David Sit Foundation to JLL. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2003",

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language = "English (US)",

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AU - Groebner, Nathan J.

AU - Frasier, Allison J.

AU - Lohr, Jamie L

N1 - Funding Information: This work was supported by an American Heart Association Fellowship (#0020507Z) to BJM; a Minnesota Medical Foundation Grant and Lillehei Heart Institute Fellowship to NJG; and a gift from the Andrew David Sit Foundation to JLL. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

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N2 - The invasion of the cardiac neural crest (CNC) into the outflow tract (OFT) and subsequent outflow tract septation are critical events during vertebrate heart development. We have performed four modified differential display screens in the chick embryo to identify genes that may be involved in CNC, OFT, secondary heart field, and heart development. The screens included differential display of RNA isolated from three different axial segments containing premigratory cranial neural crest cells; of RNA from distal outflow tract, proximal outflow tract, and atrioventricular tissue of embryonic chick hearts; and of RNA isolated from left and right cranial tissues, including the early heart fields. These screens have resulted in the identification of the five cDNA clones presented here, which are expressed in the cardiac neural crest, outflow tract and developing heart in patterns that are unique in heart development.

AB - The invasion of the cardiac neural crest (CNC) into the outflow tract (OFT) and subsequent outflow tract septation are critical events during vertebrate heart development. We have performed four modified differential display screens in the chick embryo to identify genes that may be involved in CNC, OFT, secondary heart field, and heart development. The screens included differential display of RNA isolated from three different axial segments containing premigratory cranial neural crest cells; of RNA from distal outflow tract, proximal outflow tract, and atrioventricular tissue of embryonic chick hearts; and of RNA isolated from left and right cranial tissues, including the early heart fields. These screens have resulted in the identification of the five cDNA clones presented here, which are expressed in the cardiac neural crest, outflow tract and developing heart in patterns that are unique in heart development.

KW - Cardiac chamber specification

KW - Cardiac inflow

KW - Cardiac neural crest

KW - Cardiac valve formation

KW - Chick

KW - Congenital heart disease

KW - Differential display

KW - Differential expression

KW - Heart development

KW - Linear heart tube

KW - Neural crest

KW - Notochord

KW - Outflow tract septation

KW - Secondary heart field

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Expression of cardiac neural crest and heart genes isolated by modified differential display

Abstract

Bibliographical note

Keywords

UN SDGs

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