Abstract
The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6 dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.
Original language | English (US) |
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Pages (from-to) | 319-325 |
Number of pages | 7 |
Journal | Gene |
Volume | 55 |
Issue number | 2-3 |
DOIs | |
State | Published - 1987 |
Keywords
- Recombinant DNA
- adenine methylation
- erythromycin resistance
- heterologous gene expression
- in vitro assay
- phage γ vector