Factors effecting expression of vaccines in microalgae

Raymond Surzycki; Katie Greenham; Kaoru Kitayama; Flora Dibal; Richard Wagner; Jean David Rochaix; Tarek Ajam; Stefan Surzycki

doi:10.1016/j.biologicals.2009.02.005

Factors effecting expression of vaccines in microalgae

Raymond Surzycki, Katie Greenham, Kaoru Kitayama, Flora Dibal, Richard Wagner, Jean David Rochaix, Tarek Ajam, Stefan Surzycki

Research output: Contribution to journal › Article › peer-review

158 Scopus citations

Abstract

PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.

Original language	English (US)
Pages (from-to)	133-138
Number of pages	6
Journal	Biologicals
Volume	37
Issue number	3
DOIs	https://doi.org/10.1016/j.biologicals.2009.02.005
State	Published - Jun 2009
Externally published	Yes

Bibliographical note

Funding Information:
The research was funded in part by a grant from the Indiana 21st Century Fund.

Keywords

Chlamydomonas
Chloroplast transformation
Recombinant protein expression

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title = "Factors effecting expression of vaccines in microalgae",

abstract = "PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.",

keywords = "Chlamydomonas, Chloroplast transformation, Recombinant protein expression",

author = "Raymond Surzycki and Katie Greenham and Kaoru Kitayama and Flora Dibal and Richard Wagner and Rochaix, {Jean David} and Tarek Ajam and Stefan Surzycki",

note = "Funding Information: The research was funded in part by a grant from the Indiana 21st Century Fund.",

year = "2009",

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doi = "10.1016/j.biologicals.2009.02.005",

language = "English (US)",

volume = "37",

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T1 - Factors effecting expression of vaccines in microalgae

AU - Surzycki, Raymond

AU - Greenham, Katie

AU - Kitayama, Kaoru

AU - Dibal, Flora

AU - Wagner, Richard

AU - Rochaix, Jean David

AU - Ajam, Tarek

AU - Surzycki, Stefan

N1 - Funding Information: The research was funded in part by a grant from the Indiana 21st Century Fund.

PY - 2009/6

Y1 - 2009/6

N2 - PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.

AB - PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.

KW - Chlamydomonas

KW - Chloroplast transformation

KW - Recombinant protein expression

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SN - 1045-1056

VL - 37

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EP - 138

JO - Biologicals

JF - Biologicals

IS - 3

ER -

Factors effecting expression of vaccines in microalgae

Abstract

Bibliographical note

Keywords

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