Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension

Pranav Agrawal; Nilesh P. Ingle; William S. Boyle; Emily Ward; Jakub Tolar; Kevin D. Dorfman; Theresa M. Reineke

doi:10.1021/acsami.6b01702

Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension

Pranav Agrawal, Nilesh P. Ingle, William S. Boyle, Emily Ward, Jakub Tolar, Kevin D. Dorfman, Theresa M. Reineke

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We demonstrate a highly efficient method for gene delivery into clinically relevant human cell types, such as induced pluripotent stem cells (iPSCs) and fibroblasts, reducing the protocol time by one full day. To preserve cell physiology during gene transfer, we designed a microfluidic strategy, which facilitates significant gene delivery in a short transfection time (<1 min) for several human cell types. This fast, optimized and generally applicable cell transfection method can be used for rapid screening of different delivery systems and has significant potential for high-throughput cell therapy applications.

Original language	English (US)
Pages (from-to)	8870-8874
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	8
Issue number	14
DOIs	https://doi.org/10.1021/acsami.6b01702
State	Published - Apr 27 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

Keywords

fibroblasts
flow cytometry
microfluidics
pluripotent stem cells
suspension transfection

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AU - Agrawal, Pranav

AU - Ingle, Nilesh P.

AU - Boyle, William S.

AU - Ward, Emily

AU - Tolar, Jakub

AU - Dorfman, Kevin D.

AU - Reineke, Theresa M.

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AB - We demonstrate a highly efficient method for gene delivery into clinically relevant human cell types, such as induced pluripotent stem cells (iPSCs) and fibroblasts, reducing the protocol time by one full day. To preserve cell physiology during gene transfer, we designed a microfluidic strategy, which facilitates significant gene delivery in a short transfection time (<1 min) for several human cell types. This fast, optimized and generally applicable cell transfection method can be used for rapid screening of different delivery systems and has significant potential for high-throughput cell therapy applications.

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KW - flow cytometry

KW - microfluidics

KW - pluripotent stem cells

KW - suspension transfection

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Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension

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