TY - JOUR
T1 - Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein
AU - Wu, Kai
AU - Chugh, Vinit Kumar
AU - D. Krishna, Venkatramana
AU - Wang, Yongqiang Andrew
AU - Gordon, Timothy D.
AU - Cheeran, Maxim C.J.
AU - Wang, Jian Ping
N1 - Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/12/23
Y1 - 2022/12/23
N2 - In this work, we report a 5-min magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface-functionalized magnetic nanoparticles are incubated with target analytes at 37 °C with agitation for 3 min, and the MPS reading is then taken at the fifth minute. We prove the feasibility of 5 min ultrafast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmol). Our proposed 5-min bioassay strategy may be applied to reduce the assay time for other liquid-phase, volumetric biosensors such as NMR, quantum dots, fluorescent biosensors, etc.
AB - In this work, we report a 5-min magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface-functionalized magnetic nanoparticles are incubated with target analytes at 37 °C with agitation for 3 min, and the MPS reading is then taken at the fifth minute. We prove the feasibility of 5 min ultrafast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmol). Our proposed 5-min bioassay strategy may be applied to reduce the assay time for other liquid-phase, volumetric biosensors such as NMR, quantum dots, fluorescent biosensors, etc.
KW - SARS-CoV-2
KW - bioassay
KW - magnetic nanoparticle
KW - magnetic particle spectroscopy
KW - spike protein
KW - volumetric biosensor
UR - http://www.scopus.com/inward/record.url?scp=85143977860&partnerID=8YFLogxK
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U2 - 10.1021/acsanm.2c05237
DO - 10.1021/acsanm.2c05237
M3 - Article
C2 - 36570474
AN - SCOPUS:85143977860
SN - 2574-0970
VL - 5
SP - 17503
EP - 17507
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
IS - 12
ER -
