Abstract
The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.
Original language | English (US) |
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Pages (from-to) | 3271-3275 |
Number of pages | 5 |
Journal | Biochemistry |
Volume | 21 |
Issue number | 14 |
DOIs | |
State | Published - Jul 1982 |