Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Si Yu Guo; Huub J.M. Brouwers; Rowland N. Cobbold; Joanne L. Platell; Toni A. Chapman; Vanessa R. Barrs; James R. Johnson; Darren J. Trott

doi:10.1093/jac/dks515

Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Si Yu Guo, Huub J.M. Brouwers, Rowland N. Cobbold, Joanne L. Platell, Toni A. Chapman, Vanessa R. Barrs, James R. Johnson, Darren J. Trott

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Objectives: To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. Methods: Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis. Results: The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis. Conclusions: Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.

Original language	English (US)
Article number	dks515
Pages (from-to)	1025-1031
Number of pages	7
Journal	Journal of Antimicrobial Chemotherapy
Volume	68
Issue number	5
DOIs	https://doi.org/10.1093/jac/dks515
State	Published - May 2013

Bibliographical note

Funding Information:
This study was supported by an Australian Research Council Linkage Project with Bayer Health Group (grant number LP0776358) and the New South Wales Department of Primary Industries. This material is also based on work supported by the Office of Research and Development, Medical Research Service, Department of Veterans Affairs (grant number 1 I01 CX000192 01 to J. R. J.).

Keywords

E. coli
ExPEC
Faecal carriage
Fluoroquinolone resistance
ST131

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Guo, S. Y., Brouwers, H. J. M., Cobbold, R. N., Platell, J. L., Chapman, T. A., Barrs, V. R., Johnson, J. R., & Trott, D. J. (2013). Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre. Journal of Antimicrobial Chemotherapy, 68(5), 1025-1031. Article dks515. https://doi.org/10.1093/jac/dks515

Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre. / Guo, Si Yu; Brouwers, Huub J.M.; Cobbold, Rowland N. et al.
In: Journal of Antimicrobial Chemotherapy, Vol. 68, No. 5, dks515, 05.2013, p. 1025-1031.

Research output: Contribution to journal › Article › peer-review

Guo, SY, Brouwers, HJM, Cobbold, RN, Platell, JL, Chapman, TA, Barrs, VR, Johnson, JR & Trott, DJ 2013, 'Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre', Journal of Antimicrobial Chemotherapy, vol. 68, no. 5, dks515, pp. 1025-1031. https://doi.org/10.1093/jac/dks515

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abstract = "Objectives: To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. Methods: Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis. Results: The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis. Conclusions: Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.",

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AU - Brouwers, Huub J.M.

AU - Cobbold, Rowland N.

AU - Platell, Joanne L.

AU - Chapman, Toni A.

AU - Barrs, Vanessa R.

AU - Johnson, James R.

AU - Trott, Darren J.

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N2 - Objectives: To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. Methods: Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis. Results: The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis. Conclusions: Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.

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KW - ExPEC

KW - Faecal carriage

KW - Fluoroquinolone resistance

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Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Abstract

Bibliographical note

Keywords

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