Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei; Hsu Yuan Fu; Dong Seong Cho; Dong Seong Cho; James Dutton; Wei Shou Hu; Hiroyuki Hirai; Catherine M. Verfaillie; Timothy D. O'Brien

doi:10.1016/j.scr.2017.04.010

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Haiyun Pei, Hsu Yuan Fu, Dong Seong Cho, Dong Seong Cho, James Dutton, Wei Shou Hu, Hiroyuki Hirai, Catherine M. Verfaillie, Timothy D. O'Brien

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M₃O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

Original language	English (US)
Pages (from-to)	132-136
Number of pages	5
Journal	Stem Cell Research
Volume	21
DOIs	https://doi.org/10.1016/j.scr.2017.04.010
State	Published - May 1 2017

Bibliographical note

Publisher Copyright:
© 2017

Access

10.1016/j.scr.2017.04.010

OpenUrl availability

Full text

Cite this

@article{780454268e404b48865c0a46c66bba39,

title = "Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts",

abstract = "We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.",

author = "Haiyun Pei and Fu, {Hsu Yuan} and Cho, {Dong Seong} and Cho, {Dong Seong} and James Dutton and Hu, {Wei Shou} and Hiroyuki Hirai and Verfaillie, {Catherine M.} and O'Brien, {Timothy D.}",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = may,

day = "1",

doi = "10.1016/j.scr.2017.04.010",

language = "English (US)",

volume = "21",

pages = "132--136",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

AU - Pei, Haiyun

AU - Fu, Hsu Yuan

AU - Cho, Dong Seong

AU - Dutton, James

AU - Hu, Wei Shou

AU - Hirai, Hiroyuki

AU - Verfaillie, Catherine M.

AU - O'Brien, Timothy D.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

AB - We derived a stable cell line from Chinese hamster embryonic fibroblasts by transduction of four mouse transcription factors (M3O, Sox2, Klf4, and n-Myc) using a lentiviral vector. The cell line possess all the characteristics of an induced pluripotent stem cell (iPSC) line. Given that Chinese hamster ovary (CHO) cells are the predominant host cells used for therapeutic protein production and no pluripotent stem cell line or other normal cell line has been isolated from Chinese hamster, this iPSC line may serve as a useful tool for research using CHO cells or even be used for deriving new cell lines.

UR - http://www.scopus.com/inward/record.url?scp=85018280840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018280840&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2017.04.010

DO - 10.1016/j.scr.2017.04.010

M3 - Article

C2 - 28677528

AN - SCOPUS:85018280840

SN - 1873-5061

VL - 21

SP - 132

EP - 136

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of induced pluripotent stem cells from Chinese hamster embryonic fibroblasts

Abstract

Bibliographical note

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this