Genetic approaches in the study of heparan sulfate functions in drosophila

Masahiko Takemura; Hiroshi Nakato

doi:10.1007/978-1-4939-1714-3_38

Genetic approaches in the study of heparan sulfate functions in drosophila

Masahiko Takemura, Hiroshi Nakato

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila has been a powerful system to explore the functions of HSPGs in vivo. In this chapter, we will introduce two genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting) and MARCM (mosaic analysis with a repressible cell marker).

Original language	English (US)
Pages (from-to)	497-505
Number of pages	9
Journal	Methods in Molecular Biology
Volume	1229
DOIs	https://doi.org/10.1007/978-1-4939-1714-3_38
State	Published - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

Drosophila melanogaster
GAL4/UAS
HSPG
MARCM
TARGET

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Genetic approaches in the study of heparan sulfate functions in drosophila

Abstract

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Keywords

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