Genome-Wide Association Study Identifies Two Loci for Stripe Rust Resistance in a Durum Wheat Panel from Iran

Ali Ashraf Mehrabi; Brian J. Steffenson; Alireza Pour-Aboughadareh; Oadi Matny; Mahbubjon Rahmatov

doi:10.3390/app12104963

Genome-Wide Association Study Identifies Two Loci for Stripe Rust Resistance in a Durum Wheat Panel from Iran

Ali Ashraf Mehrabi, Brian J. Steffenson, Alireza Pour-Aboughadareh, Oadi Matny, Mahbubjon Rahmatov

Plant Pathology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Stripe rust (Puccinia striiformis f. sp. tritici (Pst)) is one of the most devastating fungal diseases of durum wheat (Triticum turgidum L. var. durum Desf.). Races of Pst with new virulence combinations are emerging more regularly on wheat-growing continents, which challenges wheat breeding for resistance. This study aimed to identify and characterize resistance to Pst races based on a genome-wide association study. GWAS is an approach to analyze the associations between a genome-wide set of single-nucleotide polymorphisms (SNPs) and target phenotypic traits. A total of 139 durum wheat accessions from Iran were evaluated at the seedling stage against isolates Pstv-37 and Pstv-40 of Pst and then genotyped using a 15K SNP chip. In total, 230 significant associations were identified across 14 chromosomes, of which 30 were associated with resistance to both isolates. Furthermore, 17 durum wheat landraces showed an immune response against both Pst isolates. The SNP markers and resistant accessions identified in this study may be useful in programs breeding durum wheat for stripe rust resistance.

Original language	English (US)
Article number	4963
Journal	Applied Sciences (Switzerland)
Volume	12
Issue number	10
DOIs	https://doi.org/10.3390/app12104963
State	Published - May 1 2022

Bibliographical note

Funding Information:
This research was supported in part by the Minnesota Agricultural Experiment Station Project No. MIN-22-085. Open access funding is provided by the University of Minnesota.

Funding Information:
Funding: This research was supported in part by the Minnesota Agricultural Experiment Station Project No. MIN-22-085. Open access funding is provided by the University of Minnesota.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

GWAS
Pstv-37 and Pstv-40 isolates
SNP markers
durum wheat
stripe rust

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Genome-Wide Association Study Identifies Two Loci for Stripe Rust Resistance in a Durum Wheat Panel from Iran",

abstract = "Stripe rust (Puccinia striiformis f. sp. tritici (Pst)) is one of the most devastating fungal diseases of durum wheat (Triticum turgidum L. var. durum Desf.). Races of Pst with new virulence combinations are emerging more regularly on wheat-growing continents, which challenges wheat breeding for resistance. This study aimed to identify and characterize resistance to Pst races based on a genome-wide association study. GWAS is an approach to analyze the associations between a genome-wide set of single-nucleotide polymorphisms (SNPs) and target phenotypic traits. A total of 139 durum wheat accessions from Iran were evaluated at the seedling stage against isolates Pstv-37 and Pstv-40 of Pst and then genotyped using a 15K SNP chip. In total, 230 significant associations were identified across 14 chromosomes, of which 30 were associated with resistance to both isolates. Furthermore, 17 durum wheat landraces showed an immune response against both Pst isolates. The SNP markers and resistant accessions identified in this study may be useful in programs breeding durum wheat for stripe rust resistance.",

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note = "Funding Information: This research was supported in part by the Minnesota Agricultural Experiment Station Project No. MIN-22-085. Open access funding is provided by the University of Minnesota. Funding Information: Funding: This research was supported in part by the Minnesota Agricultural Experiment Station Project No. MIN-22-085. Open access funding is provided by the University of Minnesota. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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N2 - Stripe rust (Puccinia striiformis f. sp. tritici (Pst)) is one of the most devastating fungal diseases of durum wheat (Triticum turgidum L. var. durum Desf.). Races of Pst with new virulence combinations are emerging more regularly on wheat-growing continents, which challenges wheat breeding for resistance. This study aimed to identify and characterize resistance to Pst races based on a genome-wide association study. GWAS is an approach to analyze the associations between a genome-wide set of single-nucleotide polymorphisms (SNPs) and target phenotypic traits. A total of 139 durum wheat accessions from Iran were evaluated at the seedling stage against isolates Pstv-37 and Pstv-40 of Pst and then genotyped using a 15K SNP chip. In total, 230 significant associations were identified across 14 chromosomes, of which 30 were associated with resistance to both isolates. Furthermore, 17 durum wheat landraces showed an immune response against both Pst isolates. The SNP markers and resistant accessions identified in this study may be useful in programs breeding durum wheat for stripe rust resistance.

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Genome-Wide Association Study Identifies Two Loci for Stripe Rust Resistance in a Durum Wheat Panel from Iran

Abstract

Bibliographical note

Keywords

UN SDGs

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