Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-1 transport protein

Gregg D. Simonson; Victoria Iwanij

doi:10.1016/0378-1119(94)00824-C

Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-1 transport protein

Gregg D. Simonson, Victoria Iwanij

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We report the isolation and characterization of a rat gene (designated TI-LTP) encoding a liver-specific cell-surface glycoprotein that belongs to the type-I transporter family. This gene, including 5′ and 3′ flanking domains, was cloned from a rat λDASH genomic library and its nucleotide sequence was determined. TI-LTP is a single-copy gene which spans over 6 kb and contains nine introns ranging in size from 90 bp to over 1.8 kb. Two transcription start points were located using a nuclease S-1 protection assay. The TI-LTP promoter (pTI-LTP) lacks a canonical TATA-box element, but does contain five TAGA elements. Several putative transcription-factor-binding sites were identified in the pTI-LTP, including activator protein 2 (AP-2) sites and a peroxisome proliferator response element (PPRE).

Original language	English (US)
Pages (from-to)	243-247
Number of pages	5
Journal	Gene
Volume	154
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)00824-C
State	Published - 1995

Bibliographical note

Funding Information:
This work was supported by the NIH grant DK43794 and from American Cancer Society grant BC-688. We thank Dr. Howard Towle for many helpful suggestions during the course of this work.

Keywords

Hepatocyte
PPRE
TATA box
gene sequence
intron/exon organization

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title = "Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-1 transport protein",

abstract = "We report the isolation and characterization of a rat gene (designated TI-LTP) encoding a liver-specific cell-surface glycoprotein that belongs to the type-I transporter family. This gene, including 5′ and 3′ flanking domains, was cloned from a rat λDASH genomic library and its nucleotide sequence was determined. TI-LTP is a single-copy gene which spans over 6 kb and contains nine introns ranging in size from 90 bp to over 1.8 kb. Two transcription start points were located using a nuclease S-1 protection assay. The TI-LTP promoter (pTI-LTP) lacks a canonical TATA-box element, but does contain five TAGA elements. Several putative transcription-factor-binding sites were identified in the pTI-LTP, including activator protein 2 (AP-2) sites and a peroxisome proliferator response element (PPRE).",

keywords = "Hepatocyte, PPRE, TATA box, gene sequence, intron/exon organization",

author = "Simonson, {Gregg D.} and Victoria Iwanij",

note = "Funding Information: This work was supported by the NIH grant DK43794 and from American Cancer Society grant BC-688. We thank Dr. Howard Towle for many helpful suggestions during the course of this work.",

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AU - Simonson, Gregg D.

AU - Iwanij, Victoria

N1 - Funding Information: This work was supported by the NIH grant DK43794 and from American Cancer Society grant BC-688. We thank Dr. Howard Towle for many helpful suggestions during the course of this work.

PY - 1995

Y1 - 1995

N2 - We report the isolation and characterization of a rat gene (designated TI-LTP) encoding a liver-specific cell-surface glycoprotein that belongs to the type-I transporter family. This gene, including 5′ and 3′ flanking domains, was cloned from a rat λDASH genomic library and its nucleotide sequence was determined. TI-LTP is a single-copy gene which spans over 6 kb and contains nine introns ranging in size from 90 bp to over 1.8 kb. Two transcription start points were located using a nuclease S-1 protection assay. The TI-LTP promoter (pTI-LTP) lacks a canonical TATA-box element, but does contain five TAGA elements. Several putative transcription-factor-binding sites were identified in the pTI-LTP, including activator protein 2 (AP-2) sites and a peroxisome proliferator response element (PPRE).

AB - We report the isolation and characterization of a rat gene (designated TI-LTP) encoding a liver-specific cell-surface glycoprotein that belongs to the type-I transporter family. This gene, including 5′ and 3′ flanking domains, was cloned from a rat λDASH genomic library and its nucleotide sequence was determined. TI-LTP is a single-copy gene which spans over 6 kb and contains nine introns ranging in size from 90 bp to over 1.8 kb. Two transcription start points were located using a nuclease S-1 protection assay. The TI-LTP promoter (pTI-LTP) lacks a canonical TATA-box element, but does contain five TAGA elements. Several putative transcription-factor-binding sites were identified in the pTI-LTP, including activator protein 2 (AP-2) sites and a peroxisome proliferator response element (PPRE).

KW - Hepatocyte

KW - PPRE

KW - TATA box

KW - gene sequence

KW - intron/exon organization

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SN - 0378-1119

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EP - 247

JO - Gene

JF - Gene

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ER -

Genomic organization and promoter sequence of a gene encoding a rat liver-specific type-1 transport protein

Abstract

Bibliographical note

Keywords

Access

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