TY - JOUR
T1 - Genotype distribution of Mycoplasma hyopneumoniae in swine herds from different geographical regions
AU - dos Santos, Lucas F.
AU - Sreevatsan, Srinand
AU - Torremorell, Montserrat
AU - Moreira, Maria A.S.
AU - Sibila, Marina
AU - Pieters, Maria
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015
Y1 - 2015
N2 - Genetic heterogeneity of Mycoplasma hyopneumoniae in pigs has been reported, however there has been limited reproducibility on the molecular methods employed so far. The aim of this study was to modify and standardize a high-resolution multiple locus variable number tandem repeat analysis (MLVA), to investigate the genetic variability of M. hyopneumoniae circulating in the United States of America (USA), Brazil, Mexico and Spain. The MLVA was standardized on the basis of the number of tandem repeats in two Mycoplasma adhesins, P97 and P146, which are proteins involved in the adherence of the pathogen to cilia. A total of 355 samples obtained from the four countries were analyzed. The Simpson's diversity index for the assay was D=. 0.976 when samples from all countries were combined. A large number of MLVA types (. n=. 139) were identified, suggesting that multiple M. hyopneumoniae variants are circulating in swine. The locus P97 had 17 different types with 2-18 repeats. The P146 locus showed higher heterogeneity, with 34 different types, ranging from 7 to 48 repeats. MLVA types that presented more than 30 repeats in P146 were found in Spain and Brazil, while shorter repeats were observed in the USA and Mexico. This simplified MLVA method proved to be an efficient tool for typing M. hyopneumoniae with a high degree of stability, repeatability, and discriminatory power. In conclusion, M. hyopneumoniae showed a high variable number tandem repeat heterogeneity and this assay can be applied in molecular epidemiology investigations within farms and productions systems.
AB - Genetic heterogeneity of Mycoplasma hyopneumoniae in pigs has been reported, however there has been limited reproducibility on the molecular methods employed so far. The aim of this study was to modify and standardize a high-resolution multiple locus variable number tandem repeat analysis (MLVA), to investigate the genetic variability of M. hyopneumoniae circulating in the United States of America (USA), Brazil, Mexico and Spain. The MLVA was standardized on the basis of the number of tandem repeats in two Mycoplasma adhesins, P97 and P146, which are proteins involved in the adherence of the pathogen to cilia. A total of 355 samples obtained from the four countries were analyzed. The Simpson's diversity index for the assay was D=. 0.976 when samples from all countries were combined. A large number of MLVA types (. n=. 139) were identified, suggesting that multiple M. hyopneumoniae variants are circulating in swine. The locus P97 had 17 different types with 2-18 repeats. The P146 locus showed higher heterogeneity, with 34 different types, ranging from 7 to 48 repeats. MLVA types that presented more than 30 repeats in P146 were found in Spain and Brazil, while shorter repeats were observed in the USA and Mexico. This simplified MLVA method proved to be an efficient tool for typing M. hyopneumoniae with a high degree of stability, repeatability, and discriminatory power. In conclusion, M. hyopneumoniae showed a high variable number tandem repeat heterogeneity and this assay can be applied in molecular epidemiology investigations within farms and productions systems.
KW - Adhesins
KW - Enzootic pneumonia
KW - Molecular typing
KW - Multiple locus variable tandem analysis (MLVA)
KW - VNTR
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U2 - 10.1016/j.vetmic.2014.11.018
DO - 10.1016/j.vetmic.2014.11.018
M3 - Article
C2 - 25497236
AN - SCOPUS:84922721693
SN - 0378-1135
VL - 175
SP - 374
EP - 381
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 2-4
ER -