Giant magnetoresistance-based biosensor for detection of influenza A virus

Venkatramana D. Krishna; Kai Wu; Andres M. Perez; Jian Ping Wang

doi:10.3389/fmicb.2016.00400

Giant magnetoresistance-based biosensor for detection of influenza A virus

Venkatramana D. Krishna, Kai Wu, Andres M. Perez, Jian Ping Wang

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131 Scopus citations

Abstract

We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 10² TCID₅₀/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 10⁵ TCID₅₀/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 10³ to 10⁵ TCID₅₀/mL.

Original language	English (US)
Article number	400
Journal	Frontiers in Microbiology
Volume	7
Issue number	MAR
DOIs	https://doi.org/10.3389/fmicb.2016.00400
State	Published - Mar 29 2016

Bibliographical note

Publisher Copyright:
© 2016 Krishna, Wu, Perez and Wang.

Keywords

Biosensor
GMR chip
Giant magnetoresistance
Influenza A virus
Magnetic nanoparticle

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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abstract = "We have developed a simple and sensitive method for the detection of influenza A virus based on giant magnetoresistance (GMR) biosensor. This assay employs monoclonal antibodies to viral nucleoprotein (NP) in combination with magnetic nanoparticles (MNPs). Presence of influenza virus allows the binding of MNPs to the GMR sensor and the binding is proportional to the concentration of virus. Binding of MNPs onto the GMR sensor causes change in the resistance of sensor, which is measured in a real time electrical readout. GMR biosensor detected as low as 1.5 × 102 TCID50/mL virus and the signal intensity increased with increasing concentration of virus up to 1.0 × 105 TCID50/mL. This study showed that the GMR biosensor assay is relevant for diagnostic application since the virus concentration in nasal samples of influenza virus infected swine was reported to be in the range of 103 to 105 TCID50/mL.",

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Giant magnetoresistance-based biosensor for detection of influenza A virus

Abstract

Bibliographical note

Keywords

UN SDGs

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