TY - JOUR
T1 - Granulocyte aggregometry
T2 - A sensitive technique for the detection of C5a and complement activation
AU - Hammerschmidt, D. E.
AU - Bowers, T. K.
AU - Lammi-Keefe, C. L.
AU - Jacob, H. S.
AU - Craddock, P. R.
PY - 1980
Y1 - 1980
N2 - We have previously shown that complement (C) activated plasma causes granulocyte (PMN) aggregation in vitro and that C5a is responsible. The C-induced aggregation of PMNs treated with cytochalasin-B (CB) is markedly enhanced and irreversible, and the magnitude of the response is proportional to the log (concentration of activated plasma), allowing use of this technique to detect C5a and hence C activation. To compare the sensitivity of granulocyte aggregometry to that of more standard methods of detecting C-activation, we produced graded C-activation in vitro by treating fresh serum with varying amounts of zymosan. Aggregometry was the most sensitive index of C-activation, detecting C-activation produced by 0.02 mg zymosan/ml of serum - one tenth that required to produce C-activation detectable by C3 immunoelectrophoresis (the next most sensitive technique). Granulocyte aggregometry may also be used to detect in vivo C-activation. We have found aggregation activity in plasmas from patients with systemic lupus erythematosus, immune vasculitis, transfusion reactions, and other conditions associated with in vivo C-activation, but not in the plasmas of normal subjects.
AB - We have previously shown that complement (C) activated plasma causes granulocyte (PMN) aggregation in vitro and that C5a is responsible. The C-induced aggregation of PMNs treated with cytochalasin-B (CB) is markedly enhanced and irreversible, and the magnitude of the response is proportional to the log (concentration of activated plasma), allowing use of this technique to detect C5a and hence C activation. To compare the sensitivity of granulocyte aggregometry to that of more standard methods of detecting C-activation, we produced graded C-activation in vitro by treating fresh serum with varying amounts of zymosan. Aggregometry was the most sensitive index of C-activation, detecting C-activation produced by 0.02 mg zymosan/ml of serum - one tenth that required to produce C-activation detectable by C3 immunoelectrophoresis (the next most sensitive technique). Granulocyte aggregometry may also be used to detect in vivo C-activation. We have found aggregation activity in plasmas from patients with systemic lupus erythematosus, immune vasculitis, transfusion reactions, and other conditions associated with in vivo C-activation, but not in the plasmas of normal subjects.
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U2 - 10.1182/blood.v55.6.898.898
DO - 10.1182/blood.v55.6.898.898
M3 - Article
C2 - 6991024
AN - SCOPUS:0018828611
SN - 0006-4971
VL - 55
SP - 898
EP - 902
JO - Blood
JF - Blood
IS - 6
ER -