Abstract
A primer extension assay was used for the detection of uridine insertions occurring in vitro in synthetic pre-edited cytochrome b mRNA during incubation with a Leishmania tarentolae mitochondrial extract. Two different activities were detected that inserted uridines within the first two editing sites: one that is dependent on the secondary structure of the mRNA but is independent of both exogenous and endogenous guide RNA, and a second that does not put the same structural constraints on the mRNA, but is dependent on the presence of a cognate guide RNA.
Original language | English (US) |
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Pages (from-to) | 4212-4218 |
Number of pages | 7 |
Journal | Journal of Biological Chemistry |
Volume | 272 |
Issue number | 7 |
DOIs | |
State | Published - 1997 |