TY - JOUR
T1 - Heat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies
AU - Ijdo, Jacob W.
AU - Zhang, Yan
AU - Anderson, Matthew L.
AU - Goldberg, David
AU - Fikrig, Erol
PY - 1998
Y1 - 1998
N2 - We describe a patient with human granulocytic ehrlichiosis (HGE), a diagnosis confirmed by PCR and immunoblot analysis. Unexpectedly, immunoglobulin G (IgG) directed towards an 80-kDa ehrlichial antigen (without detectable IgM) was present in the patient's serum in the first week of illness. Lyme disease immunoblots were reactive for IgG (but not IgM), a result indicative of prior exposure to the Lyme disease spirochete. Amino- terminal sequencing revealed that the 80-kDa ehrlichial antigen was an HSP- 70 homolog similar to Borrelia burgdorferi HSP-70. We conclude that antibodies against B. burgdorferi HSP-70 may cross-react with the ehrlichial heat shock protein and that this possibility must be considered when serologic test results for HGE and Lyme disease are interpreted.
AB - We describe a patient with human granulocytic ehrlichiosis (HGE), a diagnosis confirmed by PCR and immunoblot analysis. Unexpectedly, immunoglobulin G (IgG) directed towards an 80-kDa ehrlichial antigen (without detectable IgM) was present in the patient's serum in the first week of illness. Lyme disease immunoblots were reactive for IgG (but not IgM), a result indicative of prior exposure to the Lyme disease spirochete. Amino- terminal sequencing revealed that the 80-kDa ehrlichial antigen was an HSP- 70 homolog similar to Borrelia burgdorferi HSP-70. We conclude that antibodies against B. burgdorferi HSP-70 may cross-react with the ehrlichial heat shock protein and that this possibility must be considered when serologic test results for HGE and Lyme disease are interpreted.
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U2 - 10.1128/cdli.5.1.118-120.1998
DO - 10.1128/cdli.5.1.118-120.1998
M3 - Article
C2 - 9455892
AN - SCOPUS:0031905880
SN - 1071-412X
VL - 5
SP - 118
EP - 120
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 1
ER -