Abstract
Mesp1 is a transcription factor that promotes differentiation of pluripotent cells into different mesoderm lineages including hematopoietic, cardiac and skeletal myogenic. This occurs via at least two transient cell populations: a common hematopoietic/cardiac progenitor population and a common cardiac/skeletal myogenic progenitor population. It is not established whether Mesp1-induced mesoderm cells are intrinsically heterogeneous, or are simply capable of multiple lineage decisions. In the current study, we applied single-cell RNA-seq to analyze Mesp1+ mesoderm. Initial whole transcriptome analysis showed a surprising homogeneity among Mesp1-induced mesoderm cells. However, this apparent global homogeneity masked an intrinsic heterogeneity revealed by interrogating a panel of early mesoderm patterning factors. This approach enabled discovery of subpopulations primed for hematopoietic or cardiac development. These studies demonstrate the heterogeneic nature of Mesp1+ mesoderm.
Original language | English (US) |
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Pages (from-to) | 469-475 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 474 |
Issue number | 3 |
DOIs | |
State | Published - Jun 3 2016 |
Bibliographical note
Funding Information:This work is supported by the NIH ( U01 HL100407 to M.K. and PCBC Jump Start Award PCBC_JS_2013/2_01 to S.S.K.C.).
Publisher Copyright:
© 2016 Elsevier Inc.
Keywords
- Cardiac development
- Hematopoiesis
- Mesoderm patterning
- Mesp1
- Single-cell RNA-seq