TY - JOUR
T1 - Identification and characterization of the first cholesterol-dependent cytolysins from gram-negative bacteria
AU - Hotze, Eileen M.
AU - Le, Huynh M.
AU - Sieber, Jessica R.
AU - Bruxvoort, Christina
AU - McInerney, Michael J.
AU - Tweten, Rodney K.
PY - 2013/1
Y1 - 2013/1
N2 - The cholesterol-dependent cytolysins (CDCs) are pore-forming toxins that have been exclusively associated with a wide variety of bacterial pathogens and opportunistic pathogens from theFirmicutes and Actinobacteria, which exhibit a Gram-positive type of cell structure. We have characterized the first CDCs from Gram-negative bacterial species, which include Desulfobulbus propionicus type species Widdel 1981 (DSM 2032)(desulfolysin [DLY]) and Enterobacter lignolyticu(formerly Enterobacter cloacae) SCF1 (enterolysin [ELY]). The DLY and ELY primary structuresshow that they maintain the signature motifs of the CDCs but lack an obvious secretion signal. Recombinant, purified DLY (rDLY) and ELY (rELY) exhibited cholesterol-dependent binding and cytolytic activity and formed the typical large CDC membrane oligomeric pore complex. Unlike the CDCsfrom Gram-positive species, which are human- and animal-opportunistic pathogens, neither D.propionicus nor E. lignolyticus is known to be a pathogen or commensal of humans or animals: thehabitats of both organisms appear to be restricted to anaerobic soils and/or sediments. Thesestudies reveal for the first time that the genes for functional CDCs are present in bacterial species that exhibit a Gram-negative cell structure. These are also the first bacterial species containing a CDC gene that are not known to inhabit or cause disease in humans or animals, which suggests a role of these CDCs in the defense against eukaryote bacterial predators.
AB - The cholesterol-dependent cytolysins (CDCs) are pore-forming toxins that have been exclusively associated with a wide variety of bacterial pathogens and opportunistic pathogens from theFirmicutes and Actinobacteria, which exhibit a Gram-positive type of cell structure. We have characterized the first CDCs from Gram-negative bacterial species, which include Desulfobulbus propionicus type species Widdel 1981 (DSM 2032)(desulfolysin [DLY]) and Enterobacter lignolyticu(formerly Enterobacter cloacae) SCF1 (enterolysin [ELY]). The DLY and ELY primary structuresshow that they maintain the signature motifs of the CDCs but lack an obvious secretion signal. Recombinant, purified DLY (rDLY) and ELY (rELY) exhibited cholesterol-dependent binding and cytolytic activity and formed the typical large CDC membrane oligomeric pore complex. Unlike the CDCsfrom Gram-positive species, which are human- and animal-opportunistic pathogens, neither D.propionicus nor E. lignolyticus is known to be a pathogen or commensal of humans or animals: thehabitats of both organisms appear to be restricted to anaerobic soils and/or sediments. Thesestudies reveal for the first time that the genes for functional CDCs are present in bacterial species that exhibit a Gram-negative cell structure. These are also the first bacterial species containing a CDC gene that are not known to inhabit or cause disease in humans or animals, which suggests a role of these CDCs in the defense against eukaryote bacterial predators.
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U2 - 10.1128/IAI.00927-12
DO - 10.1128/IAI.00927-12
M3 - Article
C2 - 23115036
AN - SCOPUS:84871859276
SN - 0019-9567
VL - 81
SP - 216
EP - 225
JO - Infection and immunity
JF - Infection and immunity
IS - 1
ER -