TY - JOUR
T1 - Identification and expression study of a Xenopus homologue of prenylated SNARE gene
AU - Park, Hyun Sung
AU - Kim, Myungjun
AU - Shim, Sangwoo
AU - Han, Jin Kwan
PY - 1998/7/20
Y1 - 1998/7/20
N2 - We have utilized the differential display PCR method to isolate transcripts expressed during early embryogenesis of Xenopus laevis. Among many transcripts that have been found to be expressed differentially during the development, one transcript which was expressed predominantly in the unfertilized egg, was isolated as a full-length cDNA and the sequence was determined. This cDNA contained a predicted size of 198 amino acids. A search of the GenBank database revealed that the predicted amino acid sequence of the cDNA is highly homologous - 87.8% identical - to the recently identified human protein, HsYKT6, a prenylated vesicle associated-SNARE ((soluble NSF (N-ethylmaleimide-sensitive fusion protein) attachment protein receptor)). Thus we have named the gene as Xsnare1. RT-PCR analysis showed that the Xsnare1 mRNA expressed throughout the oogenesis, in egg and in the early phase of embryogenesis and the level of expression declined after gastrulation. These results suggest that the Xsnare1, a maternally active, putative Xenopus homologue of prenylated v-SNARE, is a developmentally regulatory gene and may be play a role in the process of the early development of Xenopus laevis.
AB - We have utilized the differential display PCR method to isolate transcripts expressed during early embryogenesis of Xenopus laevis. Among many transcripts that have been found to be expressed differentially during the development, one transcript which was expressed predominantly in the unfertilized egg, was isolated as a full-length cDNA and the sequence was determined. This cDNA contained a predicted size of 198 amino acids. A search of the GenBank database revealed that the predicted amino acid sequence of the cDNA is highly homologous - 87.8% identical - to the recently identified human protein, HsYKT6, a prenylated vesicle associated-SNARE ((soluble NSF (N-ethylmaleimide-sensitive fusion protein) attachment protein receptor)). Thus we have named the gene as Xsnare1. RT-PCR analysis showed that the Xsnare1 mRNA expressed throughout the oogenesis, in egg and in the early phase of embryogenesis and the level of expression declined after gastrulation. These results suggest that the Xsnare1, a maternally active, putative Xenopus homologue of prenylated v-SNARE, is a developmentally regulatory gene and may be play a role in the process of the early development of Xenopus laevis.
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U2 - 10.1006/bbrc.1998.8957
DO - 10.1006/bbrc.1998.8957
M3 - Article
C2 - 9675119
AN - SCOPUS:0032551743
SN - 0006-291X
VL - 248
SP - 235
EP - 239
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -