Abstract
In an effort to better understand the transcriptional program regulated by transcription factor, AP-2delta, we used cDNA microarray to evaluate the relative expression of human genes in AD293 cells by exogenous expression of AP-2delta. Microarry showed 29 genes that were up-regulated and 39 genes with a down-regulated expression pattern. Among the identified genes were those encoding transcription factors, signal transduction molecules, kinases, as well as genes regulating cell growth, differentiation, and cell adhesion, a set of genes suggested to be regulated by AP-2. The results of microarray was valided for some regulated genes by real-time PCR analysis.These observations shed novel insight into the mechanism of AP-2delta action and provide a range of target genes for further investigation.
Original language | English (US) |
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Pages (from-to) | 1699-1706 |
Number of pages | 8 |
Journal | Frontiers in Bioscience |
Volume | 12 |
Issue number | 5 |
DOIs | |
State | Published - 2007 |
Externally published | Yes |
Keywords
- AP-2delta
- INF-responsive
- Microarray
- TSSK
- ZNF