Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS

Patrycja Puchalska; M. Concepción García; M. Luisa Marina

doi:10.1016/j.foodchem.2014.01.130

Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS

Patrycja Puchalska, M. Concepción García, M. Luisa Marina

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

This work evaluates, the presence of native antihypertensive peptides in five soybean-based infant formulas (SBIFs). SBIFs peptide extracts (< 10 kDa) and their sub-fractions (5-10 kDa, 3-5 kDa, and < 3 kDa) from a variety of samples were obtained by ultrafiltration and ACE inhibitory activity was determined. The highest activities were observed in the smaller (< 5 kDa) peptide fractions. A set of peptides present in various SBIFs were studied, and identified using HPLC-Q-ToF-MS. Despite ACE inhibitory activity decreasing after in vitro gastrointestinal digestion, it still remained at a high value (IC ₅₀ values of 18.2 ± 0.1 and 4.9 ± 0.1 μg/mL). Peptides resisting the action of gastrointestinal enzymes were identified and compared to previously identified peptides, highlighting the presence of peptide RPSYT. This peptide was synthesised, its antihypertensive and antioxidant activity were evaluated, and its resistance to in vitro gastrointestinal digestion and to high processing temperatures were studied.

Original language	English (US)
Pages (from-to)	62-69
Number of pages	8
Journal	Food Chemistry
Volume	157
DOIs	https://doi.org/10.1016/j.foodchem.2014.01.130
State	Published - Aug 15 2014
Externally published	Yes

Bibliographical note

Funding Information:
Authors thank the Spanish Ministry of Science and Innovation (Project CTQ2009-09022), the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER program (Project S2009/AGR-1464, ANALISYC-II), and the Spanish Ministry of Economy and Competitiveness (Project AGL2012-36362). Patrycja Puchalska thanks the University of Alcalá for her pre-doctoral contract.

Keywords

Antihypertensive activity
Bioactive peptides
HPLC
Q-ToF-MS
Soybean infant formulas

Access

10.1016/j.foodchem.2014.01.130

OpenUrl availability

Full text

Cite this

@article{380f21382e6a4cf6b8a95d16d4a94e5f,

title = "Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS",

abstract = "This work evaluates, the presence of native antihypertensive peptides in five soybean-based infant formulas (SBIFs). SBIFs peptide extracts (< 10 kDa) and their sub-fractions (5-10 kDa, 3-5 kDa, and < 3 kDa) from a variety of samples were obtained by ultrafiltration and ACE inhibitory activity was determined. The highest activities were observed in the smaller (< 5 kDa) peptide fractions. A set of peptides present in various SBIFs were studied, and identified using HPLC-Q-ToF-MS. Despite ACE inhibitory activity decreasing after in vitro gastrointestinal digestion, it still remained at a high value (IC 50 values of 18.2 ± 0.1 and 4.9 ± 0.1 μg/mL). Peptides resisting the action of gastrointestinal enzymes were identified and compared to previously identified peptides, highlighting the presence of peptide RPSYT. This peptide was synthesised, its antihypertensive and antioxidant activity were evaluated, and its resistance to in vitro gastrointestinal digestion and to high processing temperatures were studied.",

keywords = "Antihypertensive activity, Bioactive peptides, HPLC, Q-ToF-MS, Soybean infant formulas",

author = "Patrycja Puchalska and {Concepci{\'o}n Garc{\'i}a}, M. and {Luisa Marina}, M.",

note = "Funding Information: Authors thank the Spanish Ministry of Science and Innovation (Project CTQ2009-09022), the Comunidad Aut{\'o}noma of Madrid (Spain) and European funding from FEDER program (Project S2009/AGR-1464, ANALISYC-II), and the Spanish Ministry of Economy and Competitiveness (Project AGL2012-36362). Patrycja Puchalska thanks the University of Alcal{\'a} for her pre-doctoral contract. ",

year = "2014",

month = aug,

day = "15",

doi = "10.1016/j.foodchem.2014.01.130",

language = "English (US)",

volume = "157",

pages = "62--69",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS

AU - Puchalska, Patrycja

AU - Concepción García, M.

AU - Luisa Marina, M.

N1 - Funding Information: Authors thank the Spanish Ministry of Science and Innovation (Project CTQ2009-09022), the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER program (Project S2009/AGR-1464, ANALISYC-II), and the Spanish Ministry of Economy and Competitiveness (Project AGL2012-36362). Patrycja Puchalska thanks the University of Alcalá for her pre-doctoral contract.

PY - 2014/8/15

Y1 - 2014/8/15

N2 - This work evaluates, the presence of native antihypertensive peptides in five soybean-based infant formulas (SBIFs). SBIFs peptide extracts (< 10 kDa) and their sub-fractions (5-10 kDa, 3-5 kDa, and < 3 kDa) from a variety of samples were obtained by ultrafiltration and ACE inhibitory activity was determined. The highest activities were observed in the smaller (< 5 kDa) peptide fractions. A set of peptides present in various SBIFs were studied, and identified using HPLC-Q-ToF-MS. Despite ACE inhibitory activity decreasing after in vitro gastrointestinal digestion, it still remained at a high value (IC 50 values of 18.2 ± 0.1 and 4.9 ± 0.1 μg/mL). Peptides resisting the action of gastrointestinal enzymes were identified and compared to previously identified peptides, highlighting the presence of peptide RPSYT. This peptide was synthesised, its antihypertensive and antioxidant activity were evaluated, and its resistance to in vitro gastrointestinal digestion and to high processing temperatures were studied.

AB - This work evaluates, the presence of native antihypertensive peptides in five soybean-based infant formulas (SBIFs). SBIFs peptide extracts (< 10 kDa) and their sub-fractions (5-10 kDa, 3-5 kDa, and < 3 kDa) from a variety of samples were obtained by ultrafiltration and ACE inhibitory activity was determined. The highest activities were observed in the smaller (< 5 kDa) peptide fractions. A set of peptides present in various SBIFs were studied, and identified using HPLC-Q-ToF-MS. Despite ACE inhibitory activity decreasing after in vitro gastrointestinal digestion, it still remained at a high value (IC 50 values of 18.2 ± 0.1 and 4.9 ± 0.1 μg/mL). Peptides resisting the action of gastrointestinal enzymes were identified and compared to previously identified peptides, highlighting the presence of peptide RPSYT. This peptide was synthesised, its antihypertensive and antioxidant activity were evaluated, and its resistance to in vitro gastrointestinal digestion and to high processing temperatures were studied.

KW - Antihypertensive activity

KW - Bioactive peptides

KW - HPLC

KW - Q-ToF-MS

KW - Soybean infant formulas

UR - http://www.scopus.com/inward/record.url?scp=84896854619&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896854619&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2014.01.130

DO - 10.1016/j.foodchem.2014.01.130

M3 - Article

C2 - 24679752

AN - SCOPUS:84896854619

SN - 0308-8146

VL - 157

SP - 62

EP - 69

JO - Food Chemistry

JF - Food Chemistry

ER -

Identification of native angiotensin-I converting enzyme inhibitory peptides in commercial soybean based infant formulas using HPLC-Q-ToF-MS

Abstract

Bibliographical note

Keywords

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this