Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to STSs

J. Murray; J. Larsen; T. E. Michaels; A. Schaafsma; C. E. Vallejos; K. P. Pauls

doi:10.1139/g02-069

Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to STSs

J. Murray, J. Larsen, T. E. Michaels, A. Schaafsma, C. E. Vallejos, K. P. Pauls

Horticultural Science

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Abstract

A set of 79 previously mapped bean (Phaseolus vulgaris) genomic (Bng) clones were partially sequenced. BLAST database searches detected homologies between 59 of these clones and genes from a variety of plants, especially Arabidopsis thaliana. Some matches in the database to the Bng clones included a putative P-glycoprotein - ABC transporter from Arabidopsis, an early nodulin-binding protein (ENBP1) from Medicago truncatula, a lon-protease protein from spinach, a branched-chain amino-acid aminotransferase from Arabidopis, and a vacuolar sorting receptor (BP-80) from Pisum sativum. Additional matches were found for genes involved in isoprenoid biosynthesis, sulfur metabolism, proline biosynthesis, and floral development. Sequence tagged site (STSs) were produced for 16 of the clones, 2 of which contain simple sequence repeats (SSRs). Polymorphisms were detected for six of the STSs.

Original language	English (US)
Pages (from-to)	1013-1024
Number of pages	12
Journal	Genome
Volume	45
Issue number	6
DOIs	https://doi.org/10.1139/g02-069
State	Published - 2002

Keywords

CAPS
Gene identification
Molecular markers
SSR

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title = "Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to STSs",

abstract = "A set of 79 previously mapped bean (Phaseolus vulgaris) genomic (Bng) clones were partially sequenced. BLAST database searches detected homologies between 59 of these clones and genes from a variety of plants, especially Arabidopsis thaliana. Some matches in the database to the Bng clones included a putative P-glycoprotein - ABC transporter from Arabidopsis, an early nodulin-binding protein (ENBP1) from Medicago truncatula, a lon-protease protein from spinach, a branched-chain amino-acid aminotransferase from Arabidopis, and a vacuolar sorting receptor (BP-80) from Pisum sativum. Additional matches were found for genes involved in isoprenoid biosynthesis, sulfur metabolism, proline biosynthesis, and floral development. Sequence tagged site (STSs) were produced for 16 of the clones, 2 of which contain simple sequence repeats (SSRs). Polymorphisms were detected for six of the STSs.",

keywords = "CAPS, Gene identification, Molecular markers, SSR",

author = "J. Murray and J. Larsen and Michaels, {T. E.} and A. Schaafsma and Vallejos, {C. E.} and Pauls, {K. P.}",

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AU - Murray, J.

AU - Larsen, J.

AU - Michaels, T. E.

AU - Schaafsma, A.

AU - Vallejos, C. E.

AU - Pauls, K. P.

PY - 2002

Y1 - 2002

N2 - A set of 79 previously mapped bean (Phaseolus vulgaris) genomic (Bng) clones were partially sequenced. BLAST database searches detected homologies between 59 of these clones and genes from a variety of plants, especially Arabidopsis thaliana. Some matches in the database to the Bng clones included a putative P-glycoprotein - ABC transporter from Arabidopsis, an early nodulin-binding protein (ENBP1) from Medicago truncatula, a lon-protease protein from spinach, a branched-chain amino-acid aminotransferase from Arabidopis, and a vacuolar sorting receptor (BP-80) from Pisum sativum. Additional matches were found for genes involved in isoprenoid biosynthesis, sulfur metabolism, proline biosynthesis, and floral development. Sequence tagged site (STSs) were produced for 16 of the clones, 2 of which contain simple sequence repeats (SSRs). Polymorphisms were detected for six of the STSs.

AB - A set of 79 previously mapped bean (Phaseolus vulgaris) genomic (Bng) clones were partially sequenced. BLAST database searches detected homologies between 59 of these clones and genes from a variety of plants, especially Arabidopsis thaliana. Some matches in the database to the Bng clones included a putative P-glycoprotein - ABC transporter from Arabidopsis, an early nodulin-binding protein (ENBP1) from Medicago truncatula, a lon-protease protein from spinach, a branched-chain amino-acid aminotransferase from Arabidopis, and a vacuolar sorting receptor (BP-80) from Pisum sativum. Additional matches were found for genes involved in isoprenoid biosynthesis, sulfur metabolism, proline biosynthesis, and floral development. Sequence tagged site (STSs) were produced for 16 of the clones, 2 of which contain simple sequence repeats (SSRs). Polymorphisms were detected for six of the STSs.

KW - CAPS

KW - Gene identification

KW - Molecular markers

KW - SSR

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Identification of putative genes in bean (Phaseolus vulgaris) genomic (Bng) RFLP clones and their conversion to STSs

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