Immunohistochemical method for the simultaneous demonstration of three proteins in EDTA Decalcified paraffin embedded bone sections

Hermina M. Tulli; Cathy S. Carlson; Manuel J. Jayo; Larry W. Fisher; Kenneth G. Mann

doi:10.1179/his.1992.15.2.93

Immunohistochemical method for the simultaneous demonstration of three proteins in EDTA Decalcified paraffin embedded bone sections

Hermina M. Tulli, Cathy S. Carlson, Manuel J. Jayo, Larry W. Fisher, Kenneth G. Mann

Veterinary Clinical Sciences

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Abstract

Immunohistochemistry was used to demonstrate the presence of three proteins (actin, osteocalcin and alkaline phosphatase) within the same ethylenediaminetetraacetic acid (EDTA) decalcified and paraffin embedded bone tissue section. EDTA chelated calcium ions, causing endogenous alkaline phosphatase (ALP) to be inactivated. By ablating endogenous ALP activity through chelation, we were able to use an ALP based detection system before reactivation of the endogenous enzyme with magnesium chloride. After reactivation, endogenous ALP itself was demonstrated immunohistochemically. (The J Histotechnol 15:93, 1992).

Original language	English (US)
Pages (from-to)	93-97
Number of pages	5
Journal	Journal of Histotechnology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1179/his.1992.15.2.93
State	Published - Jun 1992

Bibliographical note

Funding Information:
The authors thank Dr Carolyn Moyer for her initial support in establishing our immunohistochemistry laboratory and Mrs. Karen Holbrook for manuscript preparation. Supported in part by NIH grant numbers RR00919 and HL14164

Keywords

Actin
Alkaline phosphatase
Bone
EDTA
Immunohistochemistry
Osteocalcin

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title = "Immunohistochemical method for the simultaneous demonstration of three proteins in EDTA Decalcified paraffin embedded bone sections",

abstract = "Immunohistochemistry was used to demonstrate the presence of three proteins (actin, osteocalcin and alkaline phosphatase) within the same ethylenediaminetetraacetic acid (EDTA) decalcified and paraffin embedded bone tissue section. EDTA chelated calcium ions, causing endogenous alkaline phosphatase (ALP) to be inactivated. By ablating endogenous ALP activity through chelation, we were able to use an ALP based detection system before reactivation of the endogenous enzyme with magnesium chloride. After reactivation, endogenous ALP itself was demonstrated immunohistochemically. (The J Histotechnol 15:93, 1992).",

keywords = "Actin, Alkaline phosphatase, Bone, EDTA, Immunohistochemistry, Osteocalcin",

author = "Tulli, {Hermina M.} and Carlson, {Cathy S.} and Jayo, {Manuel J.} and Fisher, {Larry W.} and Mann, {Kenneth G.}",

note = "Funding Information: The authors thank Dr Carolyn Moyer for her initial support in establishing our immunohistochemistry laboratory and Mrs. Karen Holbrook for manuscript preparation. Supported in part by NIH grant numbers RR00919 and HL14164",

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AU - Carlson, Cathy S.

AU - Jayo, Manuel J.

AU - Fisher, Larry W.

AU - Mann, Kenneth G.

N1 - Funding Information: The authors thank Dr Carolyn Moyer for her initial support in establishing our immunohistochemistry laboratory and Mrs. Karen Holbrook for manuscript preparation. Supported in part by NIH grant numbers RR00919 and HL14164

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AB - Immunohistochemistry was used to demonstrate the presence of three proteins (actin, osteocalcin and alkaline phosphatase) within the same ethylenediaminetetraacetic acid (EDTA) decalcified and paraffin embedded bone tissue section. EDTA chelated calcium ions, causing endogenous alkaline phosphatase (ALP) to be inactivated. By ablating endogenous ALP activity through chelation, we were able to use an ALP based detection system before reactivation of the endogenous enzyme with magnesium chloride. After reactivation, endogenous ALP itself was demonstrated immunohistochemically. (The J Histotechnol 15:93, 1992).

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KW - Osteocalcin

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Immunohistochemical method for the simultaneous demonstration of three proteins in EDTA Decalcified paraffin embedded bone sections

Abstract

Bibliographical note

Keywords

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