TY - JOUR
T1 - In vitro efficacy of doxorubicin and etoposide against a feline injection site sarcoma cell line
AU - Hill, Joseph
AU - Lawrence, Jessica
AU - Saba, Corey
AU - Turek, Michelle
AU - Feldhaeusser, Brittany
AU - Coutermarsh-Ott, Sheryl
AU - Barber, Jamie
AU - Smith, Jo
AU - Gogal, Robert
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014
Y1 - 2014
N2 - Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.
AB - Feline injection site sarcoma (ISS) is a locally invasive tumor, in which surgical treatment is frequently combined with radiation or chemotherapy to improve tumor control. The focus of this study was to evaluate the cytotoxic effects of doxorubicin or etoposide on a feline injection site sarcoma cell line (JB) and to assess the impact of combining these drugs on cell death and cell cycle. Both single agent and combination drug administration increased cell death and significantly reduced the number of viable cells. Cells in G0/G1 were significantly reduced while the G2/M fraction was significantly increased following treatment. Collectively, combining doxorubicin and etoposide at the lower EC yielded comparable results to the EC50 of either drug alone in degree of cytotoxicity, level of apoptosis, and % of cells in G2/M. The results of this study indicate that doxorubicin and etoposide alone and in combination differentially alter ISS cell viability and cycle.
KW - Apoptosis
KW - Cell cycle
KW - Doxorubicin
KW - Etoposide
KW - Injection site sarcoma
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U2 - 10.1016/j.rvsc.2014.07.006
DO - 10.1016/j.rvsc.2014.07.006
M3 - Article
C2 - 25085537
AN - SCOPUS:84925060611
SN - 0034-5288
VL - 97
SP - 349
EP - 357
JO - Research in veterinary science
JF - Research in veterinary science
IS - 2
ER -