TY - JOUR
T1 - Serological and molecular survey of Leptospira spp. among cart horses from an endemic area of human leptospirosis in Curitiba, Southern Brazil
AU - Finger, Mariane AngéLica
AU - de Barros Filho, Ivan Roque
AU - Leutenegger, Christian
AU - Estrada, Marko
AU - Ullmann, Leila Sabrina
AU - Langoni, Hélio
AU - Kikuti, Mariana
AU - Dornbush, Peterson Triches
AU - Deconto, Ivan
AU - Biondo, Alexander Welker
N1 - Publisher Copyright:
© 2014, Instituto de Medicina Tropical de Sao Paulo. All rights reserved.
PY - 2014
Y1 - 2014
N2 - Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.
AB - Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.
KW - Horses
KW - Leptospira spp.
KW - MAT
KW - qPCR
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U2 - 10.1590/S0036-46652014000600003
DO - 10.1590/S0036-46652014000600003
M3 - Article
C2 - 25351539
AN - SCOPUS:84908208715
SN - 0036-4665
VL - 56
SP - 473
EP - 476
JO - Revista do Instituto de Medicina Tropical de Sao Paulo
JF - Revista do Instituto de Medicina Tropical de Sao Paulo
IS - 6
ER -
