Isoform-specific dynamic translocation of PKC by α1-adrenoceptor stimulation in live cells

Jin O-Uchi; Jaime Sorenson; Bong Sook Jhun; Jyotsna Mishra; Stephen Hurst; Kaleef Williams; Shey Shing Sheu; Coeli M.B. Lopes

doi:10.1016/j.bbrc.2015.08.040

Isoform-specific dynamic translocation of PKC by α₁-adrenoceptor stimulation in live cells

Jin O-Uchi, Jaime Sorenson, Bong Sook Jhun, Jyotsna Mishra, Stephen Hurst, Kaleef Williams, Shey Shing Sheu, Coeli M.B. Lopes

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Protein kinase C (PKC) plays key roles in the regulation of signal transduction and cellular function in various cell types. At least ten PKC isoforms have been identified and intracellular localization and trafficking of these individual isoforms are important for regulation of enzyme activity and substrate specificity. PKC can be activated downstream of G_q-protein coupled receptor (G_qPCR) signaling and translocate to various cellular compartments including plasma membrane (PM). Recent reports suggested that different types of G_qPCRs would activate different PKC isoforms (classic, novel and atypical PKCs) with different trafficking patterns. However, the knowledge of isoform-specific activation of PKC by each G_qPCR is limited. α₁-Adrenoceptor (α_1-AR) is one of the G_qPCRs highly expressed in the cardiovascular system. In this study, we examined the isoform-specific dynamic translocation of PKC in living HEK293T cells by α₁-AR stimulation (α₁-ARS). Rat PKCα, βI, βII, δ, ε and ζ fused with GFP at C-term were co-transfected with human α_1A-AR into HEK293T cells. The isoform-specific dynamic translocation of PKC in living HEK293T cells by α₁-ARS using phenylephrine was measured by confocal microscopy. Before stimulation, GFP-PKCs were localized at cytosolic region. α₁-ARS strongly and rapidly translocated a classical PKC (cPKC), PKCα, (<30 s) to PM, with PKCα returning diffusively into the cytosol within 5 min. α₁-ARS rapidly translocated other cPKCs, PKCβI and PKCβII, to the PM (<30 s), with sustained membrane localization. One novel PKC (nPKC), PKCε, but not another nPKC, PKCδ, was translocated by α₁-AR stimulation to the PM (<30 s) and its membrane localization was also sustained. Finally, α₁-AR stimulation did not cause a diacylglycerol-insensitive atypical PKC, PKCζ translocation. Our data suggest that PKCα, β and ε activation may underlie physiological and pathophysiological responses of α₁-AR signaling for the phosphorylation of membrane-associated substrates including ion-channel and transporter proteins in the cardiovascular system.

Original language	English (US)
Pages (from-to)	464-470
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	465
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2015.08.040
State	Published - Sep 25 2015
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Drs. Naoaki Sato and John Hepler for providing us the plasmids of PKC-isoforms and α 1A -adrenoceptor, respectively, and Dr. Elena Fujiwara, Ms. Nobiru Suzuki, Ms. Mehreen Butt, Ms. Sarah Monaco and Mr. Michael Cypress for their technical assistance. This work was partly supported by AHA grants ( 09POST2310079 and 14BGIA18830032 to J.O.-U.), Medical Research Grant from W.W. Smith Charitable Trust , grant number: H1403 (to J.O.-U.), Foreign Study Grant Award of Kanae Foundation , 2008, No.15 (to J.O.-U.), Irisawa Memorial Promotion Award for Young Physiologists from the Physiological Society of Japan , 2010 (to J.O.-U.) and NIH grants ( 5T32AA007463-26 to S.H, R01HL093671 to S.-S.S. and R01HL114792 to C.M.B.L.).

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

Adrenoceptor
Confocal microscopy
G-protein coupled receptor
Protein kinase C
Signal transduction

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title = "Isoform-specific dynamic translocation of PKC by α1-adrenoceptor stimulation in live cells",

abstract = "Protein kinase C (PKC) plays key roles in the regulation of signal transduction and cellular function in various cell types. At least ten PKC isoforms have been identified and intracellular localization and trafficking of these individual isoforms are important for regulation of enzyme activity and substrate specificity. PKC can be activated downstream of Gq-protein coupled receptor (GqPCR) signaling and translocate to various cellular compartments including plasma membrane (PM). Recent reports suggested that different types of GqPCRs would activate different PKC isoforms (classic, novel and atypical PKCs) with different trafficking patterns. However, the knowledge of isoform-specific activation of PKC by each GqPCR is limited. α1-Adrenoceptor (α1-AR) is one of the GqPCRs highly expressed in the cardiovascular system. In this study, we examined the isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-AR stimulation (α1-ARS). Rat PKCα, βI, βII, δ, ε and ζ fused with GFP at C-term were co-transfected with human α1A-AR into HEK293T cells. The isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-ARS using phenylephrine was measured by confocal microscopy. Before stimulation, GFP-PKCs were localized at cytosolic region. α1-ARS strongly and rapidly translocated a classical PKC (cPKC), PKCα, (<30 s) to PM, with PKCα returning diffusively into the cytosol within 5 min. α1-ARS rapidly translocated other cPKCs, PKCβI and PKCβII, to the PM (<30 s), with sustained membrane localization. One novel PKC (nPKC), PKCε, but not another nPKC, PKCδ, was translocated by α1-AR stimulation to the PM (<30 s) and its membrane localization was also sustained. Finally, α1-AR stimulation did not cause a diacylglycerol-insensitive atypical PKC, PKCζ translocation. Our data suggest that PKCα, β and ε activation may underlie physiological and pathophysiological responses of α1-AR signaling for the phosphorylation of membrane-associated substrates including ion-channel and transporter proteins in the cardiovascular system.",

keywords = "Adrenoceptor, Confocal microscopy, G-protein coupled receptor, Protein kinase C, Signal transduction",

author = "Jin O-Uchi and Jaime Sorenson and Jhun, {Bong Sook} and Jyotsna Mishra and Stephen Hurst and Kaleef Williams and Sheu, {Shey Shing} and Lopes, {Coeli M.B.}",

note = "Funding Information: The authors thank Drs. Naoaki Sato and John Hepler for providing us the plasmids of PKC-isoforms and α 1A -adrenoceptor, respectively, and Dr. Elena Fujiwara, Ms. Nobiru Suzuki, Ms. Mehreen Butt, Ms. Sarah Monaco and Mr. Michael Cypress for their technical assistance. This work was partly supported by AHA grants ( 09POST2310079 and 14BGIA18830032 to J.O.-U.), Medical Research Grant from W.W. Smith Charitable Trust , grant number: H1403 (to J.O.-U.), Foreign Study Grant Award of Kanae Foundation , 2008, No.15 (to J.O.-U.), Irisawa Memorial Promotion Award for Young Physiologists from the Physiological Society of Japan , 2010 (to J.O.-U.) and NIH grants ( 5T32AA007463-26 to S.H, R01HL093671 to S.-S.S. and R01HL114792 to C.M.B.L.). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserved.",

year = "2015",

month = sep,

day = "25",

doi = "10.1016/j.bbrc.2015.08.040",

language = "English (US)",

volume = "465",

pages = "464--470",

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TY - JOUR

T1 - Isoform-specific dynamic translocation of PKC by α1-adrenoceptor stimulation in live cells

AU - O-Uchi, Jin

AU - Sorenson, Jaime

AU - Jhun, Bong Sook

AU - Mishra, Jyotsna

AU - Hurst, Stephen

AU - Williams, Kaleef

AU - Sheu, Shey Shing

AU - Lopes, Coeli M.B.

N1 - Funding Information: The authors thank Drs. Naoaki Sato and John Hepler for providing us the plasmids of PKC-isoforms and α 1A -adrenoceptor, respectively, and Dr. Elena Fujiwara, Ms. Nobiru Suzuki, Ms. Mehreen Butt, Ms. Sarah Monaco and Mr. Michael Cypress for their technical assistance. This work was partly supported by AHA grants ( 09POST2310079 and 14BGIA18830032 to J.O.-U.), Medical Research Grant from W.W. Smith Charitable Trust , grant number: H1403 (to J.O.-U.), Foreign Study Grant Award of Kanae Foundation , 2008, No.15 (to J.O.-U.), Irisawa Memorial Promotion Award for Young Physiologists from the Physiological Society of Japan , 2010 (to J.O.-U.) and NIH grants ( 5T32AA007463-26 to S.H, R01HL093671 to S.-S.S. and R01HL114792 to C.M.B.L.). Publisher Copyright: © 2015 Elsevier Inc. All rights reserved.

PY - 2015/9/25

Y1 - 2015/9/25

N2 - Protein kinase C (PKC) plays key roles in the regulation of signal transduction and cellular function in various cell types. At least ten PKC isoforms have been identified and intracellular localization and trafficking of these individual isoforms are important for regulation of enzyme activity and substrate specificity. PKC can be activated downstream of Gq-protein coupled receptor (GqPCR) signaling and translocate to various cellular compartments including plasma membrane (PM). Recent reports suggested that different types of GqPCRs would activate different PKC isoforms (classic, novel and atypical PKCs) with different trafficking patterns. However, the knowledge of isoform-specific activation of PKC by each GqPCR is limited. α1-Adrenoceptor (α1-AR) is one of the GqPCRs highly expressed in the cardiovascular system. In this study, we examined the isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-AR stimulation (α1-ARS). Rat PKCα, βI, βII, δ, ε and ζ fused with GFP at C-term were co-transfected with human α1A-AR into HEK293T cells. The isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-ARS using phenylephrine was measured by confocal microscopy. Before stimulation, GFP-PKCs were localized at cytosolic region. α1-ARS strongly and rapidly translocated a classical PKC (cPKC), PKCα, (<30 s) to PM, with PKCα returning diffusively into the cytosol within 5 min. α1-ARS rapidly translocated other cPKCs, PKCβI and PKCβII, to the PM (<30 s), with sustained membrane localization. One novel PKC (nPKC), PKCε, but not another nPKC, PKCδ, was translocated by α1-AR stimulation to the PM (<30 s) and its membrane localization was also sustained. Finally, α1-AR stimulation did not cause a diacylglycerol-insensitive atypical PKC, PKCζ translocation. Our data suggest that PKCα, β and ε activation may underlie physiological and pathophysiological responses of α1-AR signaling for the phosphorylation of membrane-associated substrates including ion-channel and transporter proteins in the cardiovascular system.

AB - Protein kinase C (PKC) plays key roles in the regulation of signal transduction and cellular function in various cell types. At least ten PKC isoforms have been identified and intracellular localization and trafficking of these individual isoforms are important for regulation of enzyme activity and substrate specificity. PKC can be activated downstream of Gq-protein coupled receptor (GqPCR) signaling and translocate to various cellular compartments including plasma membrane (PM). Recent reports suggested that different types of GqPCRs would activate different PKC isoforms (classic, novel and atypical PKCs) with different trafficking patterns. However, the knowledge of isoform-specific activation of PKC by each GqPCR is limited. α1-Adrenoceptor (α1-AR) is one of the GqPCRs highly expressed in the cardiovascular system. In this study, we examined the isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-AR stimulation (α1-ARS). Rat PKCα, βI, βII, δ, ε and ζ fused with GFP at C-term were co-transfected with human α1A-AR into HEK293T cells. The isoform-specific dynamic translocation of PKC in living HEK293T cells by α1-ARS using phenylephrine was measured by confocal microscopy. Before stimulation, GFP-PKCs were localized at cytosolic region. α1-ARS strongly and rapidly translocated a classical PKC (cPKC), PKCα, (<30 s) to PM, with PKCα returning diffusively into the cytosol within 5 min. α1-ARS rapidly translocated other cPKCs, PKCβI and PKCβII, to the PM (<30 s), with sustained membrane localization. One novel PKC (nPKC), PKCε, but not another nPKC, PKCδ, was translocated by α1-AR stimulation to the PM (<30 s) and its membrane localization was also sustained. Finally, α1-AR stimulation did not cause a diacylglycerol-insensitive atypical PKC, PKCζ translocation. Our data suggest that PKCα, β and ε activation may underlie physiological and pathophysiological responses of α1-AR signaling for the phosphorylation of membrane-associated substrates including ion-channel and transporter proteins in the cardiovascular system.

KW - Adrenoceptor

KW - Confocal microscopy

KW - G-protein coupled receptor

KW - Protein kinase C

KW - Signal transduction

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