Isolation and characterization of complementation products of Escherichia coli alkaline phosphatase

D. P. Fan; M. J. Schlesinger; A. Torriani; K. J. Barrett; C. Levinthal

doi:10.1016/S0022-2836(66)80207-3

Isolation and characterization of complementation products of Escherichia coli alkaline phosphatase

D. P. Fan, M. J. Schlesinger, A. Torriani, K. J. Barrett, C. Levinthal

Genetics, Cell Biology and Development (CBS)

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Abstract

Hybrid dimers of the Escherichia coli alkaline phosphatase were formed in vitro with one subunit each from various mutants. When one of the subunit species was labeled for density (D, ¹⁵N) and for radioactivity, it was possible to isolate the hybrids and study their properties directly. Such studies showed that some subunit species combine with greater affinity than others and that different hybrids can have different specific enzymic activities. The genetic map for the mutants studied is presented and its relationship to complementation is discussed.

Original language	English (US)
Pages (from-to)	32-48
Number of pages	17
Journal	Journal of Molecular Biology
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/S0022-2836(66)80207-3
State	Published - 1966

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AU - Fan, D. P.

AU - Schlesinger, M. J.

AU - Torriani, A.

AU - Barrett, K. J.

AU - Levinthal, C.

PY - 1966

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AB - Hybrid dimers of the Escherichia coli alkaline phosphatase were formed in vitro with one subunit each from various mutants. When one of the subunit species was labeled for density (D, 15N) and for radioactivity, it was possible to isolate the hybrids and study their properties directly. Such studies showed that some subunit species combine with greater affinity than others and that different hybrids can have different specific enzymic activities. The genetic map for the mutants studied is presented and its relationship to complementation is discussed.

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Isolation and characterization of complementation products of Escherichia coli alkaline phosphatase

Abstract

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