Isolation of 13 polymorphic microsatellite loci for slimy sculpin (Cottus cognatus)

Lorissa M. Fujishin; F. Keith Barker; David D. Huff; Loren M. Miller

doi:10.1007/s12686-009-9099-3

Isolation of 13 polymorphic microsatellite loci for slimy sculpin (Cottus cognatus)

Lorissa M. Fujishin, F. Keith Barker, David D. Huff, Loren M. Miller

Ecology, Evolution and Behavior

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Our research on slimy sculpins (Cottus cognatus), a benthic, freshwater fish, requires highly polymorphic genetic markers, and microsatellite loci developed for other Cottus species were insufficient for our needs. We therefore developed 13 polymorphic microsatellite loci from C. cognatus libraries enriched for tri- and tetranucleotide repeats. These loci had 2-22 alleles and observed heterozygosities ranging from 0.36 to 0.86 in a sample of 47 individuals from one population. There was no evidence of linkage disequilibrium; however, one locus had a putative null allele. Twelve loci also worked for mottled sculpin (Cottus bairdi) but only eight were polymorphic in a sample of seven individuals.

Original language	English (US)
Pages (from-to)	429-432
Number of pages	4
Journal	Conservation Genetics Resources
Volume	1
Issue number	1
DOIs	https://doi.org/10.1007/s12686-009-9099-3
State	Published - Dec 2009

Bibliographical note

Funding Information:
Acknowledgments This work is the result of research sponsored by the Minnesota Sea Grant Research Program supported by the NOAA office of Sea Grant, United States Department of Commerce, under grant No. R/F33-09. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 565 of the Minnesota Sea Grant College Program.

Keywords

Cottus
Enrichment
Microsatellite
Slimy sculpin

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title = "Isolation of 13 polymorphic microsatellite loci for slimy sculpin (Cottus cognatus)",

abstract = "Our research on slimy sculpins (Cottus cognatus), a benthic, freshwater fish, requires highly polymorphic genetic markers, and microsatellite loci developed for other Cottus species were insufficient for our needs. We therefore developed 13 polymorphic microsatellite loci from C. cognatus libraries enriched for tri- and tetranucleotide repeats. These loci had 2-22 alleles and observed heterozygosities ranging from 0.36 to 0.86 in a sample of 47 individuals from one population. There was no evidence of linkage disequilibrium; however, one locus had a putative null allele. Twelve loci also worked for mottled sculpin (Cottus bairdi) but only eight were polymorphic in a sample of seven individuals.",

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AU - Keith Barker, F.

AU - Huff, David D.

AU - Miller, Loren M.

N1 - Funding Information: Acknowledgments This work is the result of research sponsored by the Minnesota Sea Grant Research Program supported by the NOAA office of Sea Grant, United States Department of Commerce, under grant No. R/F33-09. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 565 of the Minnesota Sea Grant College Program.

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AB - Our research on slimy sculpins (Cottus cognatus), a benthic, freshwater fish, requires highly polymorphic genetic markers, and microsatellite loci developed for other Cottus species were insufficient for our needs. We therefore developed 13 polymorphic microsatellite loci from C. cognatus libraries enriched for tri- and tetranucleotide repeats. These loci had 2-22 alleles and observed heterozygosities ranging from 0.36 to 0.86 in a sample of 47 individuals from one population. There was no evidence of linkage disequilibrium; however, one locus had a putative null allele. Twelve loci also worked for mottled sculpin (Cottus bairdi) but only eight were polymorphic in a sample of seven individuals.

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Isolation of 13 polymorphic microsatellite loci for slimy sculpin (Cottus cognatus)

Abstract

Bibliographical note

Keywords

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