TY - JOUR
T1 - Isolation procedure and characterization of multipotent adult progenitor cells from rat bone marrow.
AU - Subramanian, Kartik
AU - Geraerts, Martine
AU - Pauwelyn, Karen A.
AU - Park, Yonsil
AU - Owens, D. Jason
AU - Muijtjens, Manja
AU - Ulloa-Montoya, Fernando
AU - Jiang, Yeuhua
AU - Verfaillie, Catherine M.
AU - Hu, Wei Shou
PY - 2010
Y1 - 2010
N2 - Multipotent adult progenitor cells (MAPCs) are adult stem cells derived from the bone marrow of mouse and rat and were described for the first time in 2002 (Jiang et al., Nature 418:41-49, 2002), and subsequently (Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896-904, 2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007). The capacity of rodent MAPC to differentiate at the single-cell level into some of the cell types of endoderm, mesoderm, and neuroectoderm germ layer lineages makes them promising candidates for the study of developmental processes. MAPC are isolated using adherent cell cultures and are selected based on morphology after a period of about 8-18 weeks. Here, we describe a step-by-step reproducible method to isolate rat MAPC from fetal and adult bone marrow. We elaborate on several aspects of the isolation protocol including, cell density and medium components, and methods for selecting and obtaining potential MAPC clones and their characterization.
AB - Multipotent adult progenitor cells (MAPCs) are adult stem cells derived from the bone marrow of mouse and rat and were described for the first time in 2002 (Jiang et al., Nature 418:41-49, 2002), and subsequently (Breyer et al., Exp Hematol 34:1596-1601, 2006; Jiang et al., Exp Hematol 30:896-904, 2002; Ulloa-Montoya et al., Genome Biol 8:R163, 2007). The capacity of rodent MAPC to differentiate at the single-cell level into some of the cell types of endoderm, mesoderm, and neuroectoderm germ layer lineages makes them promising candidates for the study of developmental processes. MAPC are isolated using adherent cell cultures and are selected based on morphology after a period of about 8-18 weeks. Here, we describe a step-by-step reproducible method to isolate rat MAPC from fetal and adult bone marrow. We elaborate on several aspects of the isolation protocol including, cell density and medium components, and methods for selecting and obtaining potential MAPC clones and their characterization.
UR - http://www.scopus.com/inward/record.url?scp=77953119443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953119443&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-691-7_4
DO - 10.1007/978-1-60761-691-7_4
M3 - Article
C2 - 20336516
AN - SCOPUS:77953119443
SN - 1064-3745
VL - 636
SP - 55
EP - 78
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -