Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells

George P. Philippidis; Janet L. Schottel; Wei Shou Hu

doi:10.1016/0141-0229(90)90022-I

Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells

George P. Philippidis, Janet L. Schottel, Wei Shou Hu

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Abstract

The kinetics of mercuric ion (Hg²⁺) reduction by Escherichia coli was studied with ether-permeabilized and intact cells. Permeabilized cells reduced Hg²⁺ 5.5 times faster than intact cells. The reduction by intact cells exhibited Michaelis-Menten kinetic behavior with respect to Hg²⁺ concentration. In contrast, the reduction by permeabilized cells followed substrate inhibition kinetics with respect to Hg²⁺ concentration and Michaelis-Menten kinetics with respect to NADPH concentration. An expression was adopted to describe the simultaneous dependence of the reduction rate of permeabilized cells on the Hg²⁺ and NADPH concentrations. Experimental data confirmed the general validity of the rate expression in a wide range of Hg²⁺ and NADPH concentrations.

Original language	English (US)
Pages (from-to)	854-859
Number of pages	6
Journal	Enzyme and Microbial Technology
Volume	12
Issue number	11
DOIs	https://doi.org/10.1016/0141-0229(90)90022-I
State	Published - Nov 1990

Bibliographical note

Funding Information:
This work was supported in part by grants from Eco-lab Inc., St Paul, Minnesota, the National Science Foundation (ECE-8552670), and the Graduate School of the University of Minnesota.

Keywords

Mercuric reduction
permeabilization
substrate inhibition

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title = "Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells",

abstract = "The kinetics of mercuric ion (Hg2+) reduction by Escherichia coli was studied with ether-permeabilized and intact cells. Permeabilized cells reduced Hg2+ 5.5 times faster than intact cells. The reduction by intact cells exhibited Michaelis-Menten kinetic behavior with respect to Hg2+ concentration. In contrast, the reduction by permeabilized cells followed substrate inhibition kinetics with respect to Hg2+ concentration and Michaelis-Menten kinetics with respect to NADPH concentration. An expression was adopted to describe the simultaneous dependence of the reduction rate of permeabilized cells on the Hg2+ and NADPH concentrations. Experimental data confirmed the general validity of the rate expression in a wide range of Hg2+ and NADPH concentrations.",

keywords = "Mercuric reduction, permeabilization, substrate inhibition",

author = "Philippidis, {George P.} and Schottel, {Janet L.} and Hu, {Wei Shou}",

note = "Funding Information: This work was supported in part by grants from Eco-lab Inc., St Paul, Minnesota, the National Science Foundation (ECE-8552670), and the Graduate School of the University of Minnesota.",

year = "1990",

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T1 - Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells

AU - Philippidis, George P.

AU - Schottel, Janet L.

AU - Hu, Wei Shou

N1 - Funding Information: This work was supported in part by grants from Eco-lab Inc., St Paul, Minnesota, the National Science Foundation (ECE-8552670), and the Graduate School of the University of Minnesota.

PY - 1990/11

Y1 - 1990/11

N2 - The kinetics of mercuric ion (Hg2+) reduction by Escherichia coli was studied with ether-permeabilized and intact cells. Permeabilized cells reduced Hg2+ 5.5 times faster than intact cells. The reduction by intact cells exhibited Michaelis-Menten kinetic behavior with respect to Hg2+ concentration. In contrast, the reduction by permeabilized cells followed substrate inhibition kinetics with respect to Hg2+ concentration and Michaelis-Menten kinetics with respect to NADPH concentration. An expression was adopted to describe the simultaneous dependence of the reduction rate of permeabilized cells on the Hg2+ and NADPH concentrations. Experimental data confirmed the general validity of the rate expression in a wide range of Hg2+ and NADPH concentrations.

AB - The kinetics of mercuric ion (Hg2+) reduction by Escherichia coli was studied with ether-permeabilized and intact cells. Permeabilized cells reduced Hg2+ 5.5 times faster than intact cells. The reduction by intact cells exhibited Michaelis-Menten kinetic behavior with respect to Hg2+ concentration. In contrast, the reduction by permeabilized cells followed substrate inhibition kinetics with respect to Hg2+ concentration and Michaelis-Menten kinetics with respect to NADPH concentration. An expression was adopted to describe the simultaneous dependence of the reduction rate of permeabilized cells on the Hg2+ and NADPH concentrations. Experimental data confirmed the general validity of the rate expression in a wide range of Hg2+ and NADPH concentrations.

KW - Mercuric reduction

KW - permeabilization

KW - substrate inhibition

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SN - 0141-0229

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SP - 854

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JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

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ER -

Kinetics of mercuric reduction in intact and permeabilized Escherichia coli cells

Abstract

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Keywords

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