Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Megan Y. Andrews; Cara M. Santelli; Owen W. Duckworth

doi:10.1016/j.mimet.2015.12.012

Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Megan Y. Andrews, Cara M. Santelli, Owen W. Duckworth

Earth and Environmental Sciences-Twin Cities

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.

Original language	English (US)
Pages (from-to)	41-43
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	121
DOIs	https://doi.org/10.1016/j.mimet.2015.12.012
State	Published - Feb 1 2016

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V.

Keywords

CAS assay
Fungi
Quantitative assay
Siderophores

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title = "Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi",

abstract = "The chrome azurol S (CAS) assay measures the chelating activity of siderophores, but its application (especially to fungi) is limited by toxicity issues. In this note, we describe a modified version of the CAS assay that is suitable for quantifying siderophore exudation for microorganisms, including fungi.",

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AU - Andrews, Megan Y.

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AU - Duckworth, Owen W.

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KW - Fungi

KW - Quantitative assay

KW - Siderophores

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Layer plate CAS assay for the quantitation of siderophore production and determination of exudation patterns for fungi

Abstract

Bibliographical note

Keywords

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