Levamisole potentiation of antigen specific lymphocyte blastogenic response in Brucella abortus exposed but nonresponsive cattle

J. M.B. Kaneene; F. C. Okino; R. K. Anderson; Charles C Muscoplat; D. W. Johnson

doi:10.1016/0165-2427(81)90040-4

Levamisole potentiation of antigen specific lymphocyte blastogenic response in Brucella abortus exposed but nonresponsive cattle

J. M.B. Kaneene, F. C. Okino, R. K. Anderson, Charles C Muscoplat, D. W. Johnson

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Abstract

Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [³H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 μg/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 μg/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle, B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.

Original language	English (US)
Pages (from-to)	75-85
Number of pages	11
Journal	Veterinary immunology and immunopathology
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/0165-2427(81)90040-4
State	Published - Feb 1981

Bibliographical note

Funding Information:
ACKNOWLEDGEMENT Supported in part by the U.S. Department of Agriculture, Veterinary Services and Science Education Administration and the Minnesota Agricultural Experiment Station. We thank Foster Soper for technical assistance.

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@article{950141331f2045c6bbdb4c3ed01bdaba,

title = "Levamisole potentiation of antigen specific lymphocyte blastogenic response in Brucella abortus exposed but nonresponsive cattle",

abstract = "Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [3H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 μg/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 μg/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle, B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.",

author = "Kaneene, {J. M.B.} and Okino, {F. C.} and Anderson, {R. K.} and Muscoplat, {Charles C} and Johnson, {D. W.}",

note = "Funding Information: ACKNOWLEDGEMENT Supported in part by the U.S. Department of Agriculture, Veterinary Services and Science Education Administration and the Minnesota Agricultural Experiment Station. We thank Foster Soper for technical assistance.",

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T1 - Levamisole potentiation of antigen specific lymphocyte blastogenic response in Brucella abortus exposed but nonresponsive cattle

AU - Kaneene, J. M.B.

AU - Okino, F. C.

AU - Anderson, R. K.

AU - Muscoplat, Charles C

AU - Johnson, D. W.

N1 - Funding Information: ACKNOWLEDGEMENT Supported in part by the U.S. Department of Agriculture, Veterinary Services and Science Education Administration and the Minnesota Agricultural Experiment Station. We thank Foster Soper for technical assistance.

PY - 1981/2

Y1 - 1981/2

N2 - Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [3H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 μg/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 μg/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle, B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.

AB - Incubation of Brucella abortus (field strain) infected and strain 19 vaccinated bovine peripheral blood lymphocytes with B. abortus antigen and levamisole caused a consistently significant increase in [3H] thymidine uptake when compared to cultures without levamisole. Levamisole did not potentiate B. abortus-induced blastogenic response of lymphocytes from non-exposed cattle. A dose response study showed that 10 μg/culture induced maximum potentiation of B. abortus-induced lymphocyte stimulation. Using the 10 μg/well concentration of levamisole, further studies were conducted to determine the net potentiation of the blastogenic responses in lymphocytes from B. abortus (field strain) infected cattle, B. abortus strain 19 vaccinated but nonresponsive and non-exposed cattle. Levamisole significantly potentiated the B. abortus-induced lymphocyte blastogenesis in lymphocytes from unresponsive cattle.

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IS - 1

ER -

Levamisole potentiation of antigen specific lymphocyte blastogenic response in Brucella abortus exposed but nonresponsive cattle

Abstract

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