Mapping a novel black spot resistance locus in the climbing rose brite eyes™ (‘radbrite’)

Jason D. Zurn; David C. Zlesak; Matthew Holen; James M. Bradeen; Stan C. Hokanson; Nahla V. Bassil

doi:10.3389/fpls.2018.01730

Mapping a novel black spot resistance locus in the climbing rose brite eyes™ (‘radbrite’)

Jason D. Zurn, David C. Zlesak, Matthew Holen, James M. Bradeen, Stan C. Hokanson, Nahla V. Bassil

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Rose black spot, caused by Diplocarpon rosae, is one of the most devastating foliar diseases of cultivated roses (Rosa spp.). The globally distributed pathogen has the potential to cause large economic losses in the outdoor cultivation of roses. Fungicides are the primary method to manage the disease, but are often viewed unfavorably by home gardeners due to potential environmental and health impacts. As such, rose cultivars with genetic resistance to black spot are highly desired. The tetraploid climbing rose Brite Eyes™ (‘RADbrite’) is known for its resistance to black spot. To better characterize the resistance present in Brite Eyes™, phenotyping was conducted on a 94 individual F₁ population developed by crossing Brite Eyes™ to the susceptible tetraploid rose ‘Morden Blush’. Brite Eyes™ was resistant to all D. rosae races evaluated except for race 12. The progeny were either resistant or susceptible to all races (2, 3, 8, 9, 10, 11, and 13) evaluated. The segregation ratio was 1:1 (χ² = 0.3830, P = 0.5360) suggesting resistance is conferred by a single locus. The roses were genotyped with the WagRhSNP 68K Axiom array and the ‘polymapR’ package was used to construct a map. A single resistance locus (Rdr4) was identified on the long arm of chromosome 5 homoeolog 4. Three resistance loci have been previously identified (Rdr1, Rdr2, and Rdr3). Both Rdr1 and Rdr2 are located on a chromosome 1 homoeolog. The chromosomal location of Rdr3 is unknown, however, races 3 and 9 are virulent on Rdr3. Rdr4 is either a novel gene or an allele of Rdr3 as it provides resistance to races 3 and 9. Due to its broad resistance, Rdr4 is an excellent gene to introgress into new rose cultivars.

Original language	English (US)
Article number	1730
Journal	Frontiers in Plant Science
Volume	871
DOIs	https://doi.org/10.3389/fpls.2018.01730
State	Published - 2018

Bibliographical note

Funding Information:
The authors would like to thank Christina Mulch, April Nyberg, Sarah Kummeth, Drew Zagala, and Seth Heder for their technical support and Bailey Nurseries and Star® Roses and Plants for donating rose plants. Funding. This research was funded through the USDA’s National Institute of Food and Agriculture – Specialty Crop Research Initiative project, ‘RosBREED: Combining Disease Resistance and Horticultural Quality in New Rosaceous Cultivars’ (2014-51181-22378).

Publisher Copyright:
© 2018 Zurn, Zlesak, Holen, Bradeen, Hokanson and Bassil.

Keywords

Host-resistance
Ornamental plants
Polyploid
RosBREED
SNP

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title = "Mapping a novel black spot resistance locus in the climbing rose brite eyes{\texttrademark} ({\textquoteleft}radbrite{\textquoteright})",

abstract = "Rose black spot, caused by Diplocarpon rosae, is one of the most devastating foliar diseases of cultivated roses (Rosa spp.). The globally distributed pathogen has the potential to cause large economic losses in the outdoor cultivation of roses. Fungicides are the primary method to manage the disease, but are often viewed unfavorably by home gardeners due to potential environmental and health impacts. As such, rose cultivars with genetic resistance to black spot are highly desired. The tetraploid climbing rose Brite Eyes{\texttrademark} ({\textquoteleft}RADbrite{\textquoteright}) is known for its resistance to black spot. To better characterize the resistance present in Brite Eyes{\texttrademark}, phenotyping was conducted on a 94 individual F1 population developed by crossing Brite Eyes{\texttrademark} to the susceptible tetraploid rose {\textquoteleft}Morden Blush{\textquoteright}. Brite Eyes{\texttrademark} was resistant to all D. rosae races evaluated except for race 12. The progeny were either resistant or susceptible to all races (2, 3, 8, 9, 10, 11, and 13) evaluated. The segregation ratio was 1:1 (χ2 = 0.3830, P = 0.5360) suggesting resistance is conferred by a single locus. The roses were genotyped with the WagRhSNP 68K Axiom array and the {\textquoteleft}polymapR{\textquoteright} package was used to construct a map. A single resistance locus (Rdr4) was identified on the long arm of chromosome 5 homoeolog 4. Three resistance loci have been previously identified (Rdr1, Rdr2, and Rdr3). Both Rdr1 and Rdr2 are located on a chromosome 1 homoeolog. The chromosomal location of Rdr3 is unknown, however, races 3 and 9 are virulent on Rdr3. Rdr4 is either a novel gene or an allele of Rdr3 as it provides resistance to races 3 and 9. Due to its broad resistance, Rdr4 is an excellent gene to introgress into new rose cultivars.",

keywords = "Host-resistance, Ornamental plants, Polyploid, RosBREED, SNP",

author = "Zurn, {Jason D.} and Zlesak, {David C.} and Matthew Holen and Bradeen, {James M.} and Hokanson, {Stan C.} and Bassil, {Nahla V.}",

note = "Funding Information: The authors would like to thank Christina Mulch, April Nyberg, Sarah Kummeth, Drew Zagala, and Seth Heder for their technical support and Bailey Nurseries and Star{\textregistered} Roses and Plants for donating rose plants. Funding. This research was funded through the USDA{\textquoteright}s National Institute of Food and Agriculture – Specialty Crop Research Initiative project, {\textquoteleft}RosBREED: Combining Disease Resistance and Horticultural Quality in New Rosaceous Cultivars{\textquoteright} (2014-51181-22378). Publisher Copyright: {\textcopyright} 2018 Zurn, Zlesak, Holen, Bradeen, Hokanson and Bassil.",

year = "2018",

doi = "10.3389/fpls.2018.01730",

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AU - Holen, Matthew

AU - Bradeen, James M.

AU - Hokanson, Stan C.

AU - Bassil, Nahla V.

N1 - Funding Information: The authors would like to thank Christina Mulch, April Nyberg, Sarah Kummeth, Drew Zagala, and Seth Heder for their technical support and Bailey Nurseries and Star® Roses and Plants for donating rose plants. Funding. This research was funded through the USDA’s National Institute of Food and Agriculture – Specialty Crop Research Initiative project, ‘RosBREED: Combining Disease Resistance and Horticultural Quality in New Rosaceous Cultivars’ (2014-51181-22378). Publisher Copyright: © 2018 Zurn, Zlesak, Holen, Bradeen, Hokanson and Bassil.

PY - 2018

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N2 - Rose black spot, caused by Diplocarpon rosae, is one of the most devastating foliar diseases of cultivated roses (Rosa spp.). The globally distributed pathogen has the potential to cause large economic losses in the outdoor cultivation of roses. Fungicides are the primary method to manage the disease, but are often viewed unfavorably by home gardeners due to potential environmental and health impacts. As such, rose cultivars with genetic resistance to black spot are highly desired. The tetraploid climbing rose Brite Eyes™ (‘RADbrite’) is known for its resistance to black spot. To better characterize the resistance present in Brite Eyes™, phenotyping was conducted on a 94 individual F1 population developed by crossing Brite Eyes™ to the susceptible tetraploid rose ‘Morden Blush’. Brite Eyes™ was resistant to all D. rosae races evaluated except for race 12. The progeny were either resistant or susceptible to all races (2, 3, 8, 9, 10, 11, and 13) evaluated. The segregation ratio was 1:1 (χ2 = 0.3830, P = 0.5360) suggesting resistance is conferred by a single locus. The roses were genotyped with the WagRhSNP 68K Axiom array and the ‘polymapR’ package was used to construct a map. A single resistance locus (Rdr4) was identified on the long arm of chromosome 5 homoeolog 4. Three resistance loci have been previously identified (Rdr1, Rdr2, and Rdr3). Both Rdr1 and Rdr2 are located on a chromosome 1 homoeolog. The chromosomal location of Rdr3 is unknown, however, races 3 and 9 are virulent on Rdr3. Rdr4 is either a novel gene or an allele of Rdr3 as it provides resistance to races 3 and 9. Due to its broad resistance, Rdr4 is an excellent gene to introgress into new rose cultivars.

AB - Rose black spot, caused by Diplocarpon rosae, is one of the most devastating foliar diseases of cultivated roses (Rosa spp.). The globally distributed pathogen has the potential to cause large economic losses in the outdoor cultivation of roses. Fungicides are the primary method to manage the disease, but are often viewed unfavorably by home gardeners due to potential environmental and health impacts. As such, rose cultivars with genetic resistance to black spot are highly desired. The tetraploid climbing rose Brite Eyes™ (‘RADbrite’) is known for its resistance to black spot. To better characterize the resistance present in Brite Eyes™, phenotyping was conducted on a 94 individual F1 population developed by crossing Brite Eyes™ to the susceptible tetraploid rose ‘Morden Blush’. Brite Eyes™ was resistant to all D. rosae races evaluated except for race 12. The progeny were either resistant or susceptible to all races (2, 3, 8, 9, 10, 11, and 13) evaluated. The segregation ratio was 1:1 (χ2 = 0.3830, P = 0.5360) suggesting resistance is conferred by a single locus. The roses were genotyped with the WagRhSNP 68K Axiom array and the ‘polymapR’ package was used to construct a map. A single resistance locus (Rdr4) was identified on the long arm of chromosome 5 homoeolog 4. Three resistance loci have been previously identified (Rdr1, Rdr2, and Rdr3). Both Rdr1 and Rdr2 are located on a chromosome 1 homoeolog. The chromosomal location of Rdr3 is unknown, however, races 3 and 9 are virulent on Rdr3. Rdr4 is either a novel gene or an allele of Rdr3 as it provides resistance to races 3 and 9. Due to its broad resistance, Rdr4 is an excellent gene to introgress into new rose cultivars.

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KW - Ornamental plants

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ER -

Mapping a novel black spot resistance locus in the climbing rose brite eyes™ (‘radbrite’)

Abstract

Bibliographical note

Keywords

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