TY - JOUR
T1 - Mapping action potentials and calcium transients simultaneously from the intact heart
AU - Laurita, Kenneth R.
AU - Singal, Ashish
PY - 2001/5
Y1 - 2001/5
N2 - Intracellular calcium handling plays an important role in cardiac electrophysiology. Using two fluorescent indicators, we developed an optical mapping system that is capable of measuring calcium transients and action potentials at 256 recording sites simultaneously from the intact guinea pig heart. On the basis of in vitro measurements of dye excitation and emission spectra, excitation and emission filters at 515 ± 5 and >695 nm, respectively, were used to measure action potentials with di-4-ANEPPS, and excitation and emission filters at 365 ± 25 and 485 ± 5 nm, respectively, were used to measure calcium transients with indo 1. The percent error due to spectral overlap was small when action potentials were measured (1.7 ± 1.0%, n = 3) and negligible when calcium transients were measured (0%, n = 3). Recordings of calcium transients, action potentials, and isochrone maps of depolarization time and the time of calcium transient onset indicated negligible error due to fluorescence emission overlap. These data demonstrate that the error due to spectral overlap of indo 1 and di-4-ANEPPS is sufficiently small, such that optical mapping techniques can be used to measure calcium transients and action potentials simultaneously in the intact heart.
AB - Intracellular calcium handling plays an important role in cardiac electrophysiology. Using two fluorescent indicators, we developed an optical mapping system that is capable of measuring calcium transients and action potentials at 256 recording sites simultaneously from the intact guinea pig heart. On the basis of in vitro measurements of dye excitation and emission spectra, excitation and emission filters at 515 ± 5 and >695 nm, respectively, were used to measure action potentials with di-4-ANEPPS, and excitation and emission filters at 365 ± 25 and 485 ± 5 nm, respectively, were used to measure calcium transients with indo 1. The percent error due to spectral overlap was small when action potentials were measured (1.7 ± 1.0%, n = 3) and negligible when calcium transients were measured (0%, n = 3). Recordings of calcium transients, action potentials, and isochrone maps of depolarization time and the time of calcium transient onset indicated negligible error due to fluorescence emission overlap. These data demonstrate that the error due to spectral overlap of indo 1 and di-4-ANEPPS is sufficiently small, such that optical mapping techniques can be used to measure calcium transients and action potentials simultaneously in the intact heart.
KW - Di-4-ANEPPS
KW - Electrophysiology
KW - Indo 1
KW - Intracellular calcium
KW - Optical mapping
UR - http://www.scopus.com/inward/record.url?scp=0035036296&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035036296&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.2001.280.5.h2053
DO - 10.1152/ajpheart.2001.280.5.h2053
M3 - Article
C2 - 11299206
AN - SCOPUS:0035036296
SN - 0363-6135
VL - 280
SP - H2053-H2060
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 5 49-5
ER -